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Up to three distinct complement regulator-acquiring surface proteins (CRASPs) may be expressed by serum-resistant<jats:italic>B. spielmanii<\/jats:italic>, each exhibiting an affinity for CFH and\/or CFHL1. Here, we describe the functional characterization of the 15-kDa CRASPs of<jats:italic>B. spielmanii<\/jats:italic>, members of the polymorphic Erp (OspE\/F-related) protein family, that bind two distinct host complement regulators, CFH and factor H-related protein 1 (CFHR1), but not CFHL1. CFH bound to the<jats:italic>B. spielmanii<\/jats:italic>CRASPs maintained cofactor activity for factor I-mediated C3b inactivation. Three naturally occurring alleles of this protein bound CFH and CFHR1 while a fourth natural allele could not. Comparative sequence analysis of these protein alleles identified a single amino acid, histidine-79, as playing a significant role in CFH\/CFHR1 binding, with substitution by an arginine completely abrogating ligand binding. The mutation of His-79 to Arg did not inhibit binding of plasminogen, another known ligand of this group of borrelial outer-surface proteins.<\/jats:p>","DOI":"10.1128\/iai.00691-09","type":"journal-article","created":{"date-parts":[[2009,10,27]],"date-time":"2009-10-27T01:29:57Z","timestamp":1256606997000},"page":"39-48","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Functional Characterization of<i>Borrelia spielmanii<\/i>Outer Surface Proteins That Interact with Distinct Members of the Human Factor H Protein Family and with Plasminogen"],"prefix":"10.1128","volume":"78","author":[{"given":"Annekatrin","family":"Seling","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Paul Ehrlich Strasse 40, D-60596 Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinna","family":"Siegel","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Paul Ehrlich Strasse 40, D-60596 Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt fu\u0308r Gesundheit und Lebensmittelsicherheit, Veterina\u0308rstrasse 2, D-85764 Oberschlei\u00dfheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon L.","family":"Jutras","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine A.","family":"Brissette","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Skerka","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Beutenbergstrasse 11a, D-07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Wallich","sequence":"additional","affiliation":[{"name":"Institute for Immunology, University Hospital of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Zipfel","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Beutenbergstrasse 11a, D-07745 Jena, Germany"},{"name":"Friedrich Schiller University, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kraiczy","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Paul Ehrlich Strasse 40, D-60596 Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6195"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200526354"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3847"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3685-3691.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112072200"},{"key":"e_1_3_2_7_2","first-page":"1","volume":"57","year":"2008","unstructured":"Bacon, R. 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