{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T02:54:17Z","timestamp":1769741657498,"version":"3.49.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000072","name":"HHS | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE012768"],"award-info":[{"award-number":["R01DE012768"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"HHS | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["T90DE21984"],"award-info":[{"award-number":["T90DE21984"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ability of the subgingival microbial community to induce an inappropriate inflammatory response ultimately results in the destruction of bone and gingival tissue. In this study, subgingival plaque samples from both healthy and diseased sites in the same individual were obtained from adults with chronic periodontitis and screened for their ability to either activate Toll-like receptor 2 (TLR2) or TLR4 and to antagonize TLR4-specific activation by agonist,\n            <jats:named-content content-type=\"genus-species\">Fusobacterium nucleatum<\/jats:named-content>\n            LPS. Subgingival plaque from diseased sites strongly activated TLR4, whereas matched plaque samples obtained from healthy sites were significantly more variable, with some samples displaying strong TLR4 antagonism, while others were strong TLR4 agonists when combined with\n            <jats:named-content content-type=\"genus-species\">F. nucleatum<\/jats:named-content>\n            LPS. Similar results were observed when TLR4 dependent E-selectin expression by endothelial cells was determined. These results are the first to demonstrate TLR4 antagonism from human plaque samples and demonstrate that healthy but not diseased sites display a wide variation in TLR4 agonist and antagonist behavior. The results have identified a novel characteristic of clinically healthy sites and warrant further study on the contribution of TLR4 antagonism in the progression of a healthy periodontal site to a diseased one.\n          <\/jats:p>","DOI":"10.1128\/iai.00693-15","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T12:29:14Z","timestamp":1445344154000},"page":"120-126","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Subgingival Plaque in Periodontal Health Antagonizes at Toll-Like Receptor 4 and Inhibits E-Selectin Expression on Endothelial Cells"],"prefix":"10.1128","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7416-6201","authenticated-orcid":false,"given":"Thao T.","family":"To","sequence":"first","affiliation":[{"name":"Department of Periodontics, University of Washington School of Dentistry, Seattle, Washington, USA"},{"name":"Department of Oral Health Sciences, University of Washington School of Dentistry, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pinar","family":"G\u00fcm\u00fc\u015f","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Ege University, \u0130zmir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nejat","family":"Nizam","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Ege University, \u0130zmir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurcan","family":"Buduneli","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Ege University, \u0130zmir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard P.","family":"Darveau","sequence":"additional","affiliation":[{"name":"Department of Periodontics, University of Washington School of Dentistry, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.37"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027009648.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00190.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2009.04.015"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00937-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01702.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6799-6807.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1623-1632.1999"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4490"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11.1950"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.831-838.1983"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/096805199400100202"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00859.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00672.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058496"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01349.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1039\/b000368i"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01036-12"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.863-866.2003"},{"key":"e_1_3_2_23_2","volume-title":"Applied statistics for the behavioral sciences","author":"Hinkle DE","year":"2003","unstructured":"Hinkle DE, Wiersma W, Jurs SG. 2003. 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