{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T01:48:03Z","timestamp":1794016083342,"version":"4.8.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n            accounts for 20 to 30% of all community-acquired pneumonia and has been associated with other airway pathologies, including asthma, and a range of extrapulmonary manifestations. Although the entire genomic sequence of\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            has been completed, the functions of many of these genes in mycoplasma physiology are unknown. In this study, we focused on\n            <jats:italic>clpB<\/jats:italic>\n            , a well-known heat shock gene in other bacteria, to examine its role in mycoplasma growth. Transcriptional and translational analyses of heat shock in\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            indicated that\n            <jats:italic>clpB<\/jats:italic>\n            is significantly upregulated, reinforcing its status as a critical responder to heat stress. Interestingly,\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            ClpB does not use dual translational start points for ClpB synthesis, like other ClpB-characterized bacteria. Biochemical characterization of purified\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            recombinant ClpB revealed casein- and lysine-independent ATPase activity and DnaK-DnaJ-GrpE-dependent chaperone activity. An\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            mini-Tn\n            <jats:italic>4001<\/jats:italic>\n            -integrated,\n            <jats:italic>clpB<\/jats:italic>\n            -null mutant was impaired in its ability to replicate under permissive growth conditions, demonstrating the growth-promoting status of ClpB.\n          <\/jats:p>","DOI":"10.1128\/iai.00698-08","type":"journal-article","created":{"date-parts":[[2008,9,9]],"date-time":"2008-09-09T00:33:56Z","timestamp":1220920436000},"page":"5082-5092","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Characterization of a Unique ClpB Protein of\n            <i>Mycoplasma pneumoniae<\/i>\n            and Its Impact on Growth"],"prefix":"10.1128","volume":"76","author":[{"given":"T. R.","family":"Kannan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Texas 78229-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oxana","family":"Musatovova","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Texas 78229-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramod","family":"Gowda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, Texas 78229-3900"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel B.","family":"Baseman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The 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