{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:22:05Z","timestamp":1792624925839,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vaccination with the recombinant N terminus of the candidal adhesin Als3p (rAls3p-N) protects mice from lethal candidemia. Candidal Als3p also is structurally similar to the microbial surface components recognizing adhesive matrix molecule adhesin, clumping factor, from\n                    <jats:italic>Staphylococcus aureus.<\/jats:italic>\n                    To determine the potential for cross-kingdom vaccination, we immunized mice with rAls3p-N or negative control proteins and challenged them via the tail vein with\n                    <jats:italic>S. aureus<\/jats:italic>\n                    or other gram-positive or gram-negative pathogens. The rAls3p-N vaccine, but neither tetanus toxoid nor a related Als protein (Als5p), improved the survival of vaccinated mice subsequently infected with multiple clinical isolates of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , including methicillin-resistant strains. The rAls3p-N vaccine was effective against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    when combined with aluminum hydroxide adjuvant. However, the vaccine did not improve the survival of mice infected with other bacterial pathogens. Vaccinated, infected mice mounted moderated type 1 immune responses. T lymphocyte-deficient mice were more susceptible to\n                    <jats:italic>S. aureus<\/jats:italic>\n                    infection, but B lymphocyte-deficient mice were not. Furthermore, T but not B lymphocytes from vaccinated mice mediated protection in adoptive transfer studies. The passive transfer of immune serum was not protective. These data provide the foundation for cross-kingdom vaccine development against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>Candida<\/jats:italic>\n                    , which collectively cause 200,000 bloodstream infections resulting in \u226540,000 to 50,000 deaths annually in the United States alone.\n                  <\/jats:p>","DOI":"10.1128\/iai.00700-08","type":"journal-article","created":{"date-parts":[[2008,7,21]],"date-time":"2008-07-21T20:45:10Z","timestamp":1216673110000},"page":"4574-4580","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":130,"title":["The Antifungal Vaccine Derived from the Recombinant N Terminus of Als3p Protects Mice against the Bacterium\n                    <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Brad","family":"Spellberg","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"},{"name":"The David Geffen School of Medicine at UCLA, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf S.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"},{"name":"The David Geffen School of Medicine at UCLA, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Yeaman","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"},{"name":"The David Geffen School of Medicine at UCLA, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Fu","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"},{"name":"The David Geffen School of Medicine at UCLA, Los 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Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 West Carson St., Torrance, California 90502"},{"name":"The David Geffen School of Medicine at UCLA, Los Angeles, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Lipke","sequence":"additional","affiliation":[{"name":"Department of Biology, Brooklyn College of City University of New York, Brooklyn, New York 11210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Otoo","sequence":"additional","affiliation":[{"name":"Department of Biology, Brooklyn College of City University of New York, Brooklyn, New York 11210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"John E.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor\u2014University of California at Los Angeles (UCLA) Medical Center, 1124 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