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To circumvent this problem, we injected CpG DNA admixed to the immunodominant p60<jats:sub>217-225<\/jats:sub>peptide from<jats:italic>Listeria monocytogenes<\/jats:italic>subcutaneously into BALB\/c mice and coadministered a p60-translocating<jats:italic>Salmonella<\/jats:italic>strain by the orogastric route. The distribution of tetramer-positive p60<jats:sub>217-225<\/jats:sub>-specific effector and memory CD8 T cells was analyzed by costaining of lymphocytes with CD62L and CD127. In contrast to the single oral application of recombinant<jats:italic>Salmonella<\/jats:italic>or single immunization with CpG and p60, in the spleens from mice immunized with a combination of both vaccine types a significantly higher level of p60-specific CD8 T cells with a predominance of the effector memory T-cell subset was detected. In vivo protection studies revealed that this CD8 T-cell population conferred sterile protective immunity against a lethal infection with<jats:italic>L. monocytogenes<\/jats:italic>. However, p60-specific central memory CD8 T cells induced by single vaccination with CpG and p60 were not able confer effective protection against rapidly replicating intracellular<jats:italic>Listeria<\/jats:italic>. In conclusion, we provide compelling evidence that the combination of<jats:italic>Salmonella<\/jats:italic>type III-mediated antigen delivery and CpG immunization is an attractive novel vaccination strategy to modulate CD8 differentiation patterns toward distinct antigen-specific T-cell subsets with favorable protective capacities.<\/jats:p>","DOI":"10.1128\/iai.00700-09","type":"journal-article","created":{"date-parts":[[2009,9,22]],"date-time":"2009-09-22T01:48:31Z","timestamp":1253584111000},"page":"5501-5508","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Superior Protective Immunity against Murine Listeriosis by Combined Vaccination with CpG DNA and Recombinant<i>Salmonella enterica<\/i>Serovar Typhimurium"],"prefix":"10.1128","volume":"77","author":[{"given":"Christina","family":"Berchtold","sequence":"first","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Panthel","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Jellbauer","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Ko\u0308hn","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Roider","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Partilla","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u0308rgen","family":"Heesemann","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Endres","sequence":"additional","affiliation":[{"name":"Center of Integrated Protein Science CIPS-M at the Division of Clinical Pharmacology, Department of Internal Medicine, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole","family":"Bourquin","sequence":"additional","affiliation":[{"name":"Center of Integrated Protein Science CIPS-M at the Division of Clinical Pharmacology, Department of Internal Medicine, Ludwig Maximilians-University Munich, 80336 Munich"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Ru\u0308ssmann","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians-University Munich, 80336 Munich"},{"name":"HELIOS Clinic Emil von Behring, Institute for Microbiology, Immunology, and Laboratory Medicine, 14165 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/90609"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4677"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4686"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","volume":"139","year":"1987","unstructured":"Bishop, D. 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