{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:58:59Z","timestamp":1791093539473,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which\n            <jats:italic>ospD<\/jats:italic>\n            is deleted, we demonstrate that this protein is not required for\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            survival and infectivity in either the mouse or the tick. However, examination of both transcript levels and protein expression indicates that OspD expression is limited to a discrete window of time during\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            replication within the tick. This time frame corresponds to tick detachment from the host following feeding, and expression of OspD continues during tick digestion of the blood meal but is low or undetectable after the tick has molted. The high level of OspD production correlates to the highest cell densities that\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            is known to reach in vivo. Although OspD is nonessential to the infectious cycle of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            , the tight regulation of expression suggests a beneficial contribution of OspD to the spirochete during bacterial replication within the tick midgut.\n          <\/jats:p>","DOI":"10.1128\/iai.00714-07","type":"journal-article","created":{"date-parts":[[2008,3,11]],"date-time":"2008-03-11T01:05:47Z","timestamp":1205197547000},"page":"1970-1978","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["A Tightly Regulated Surface Protein of\n            <i>Borrelia burgdorferi<\/i>\n            Is Not Essential to the Mouse-Tick Infectious Cycle"],"prefix":"10.1128","volume":"76","author":[{"given":"Philip E.","family":"Stewart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Bestor","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St., Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonah N.","family":"Cullen","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St., Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St., Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.2.394-396.1986"},{"key":"e_1_3_2_3_2","first-page":"627","volume":"57","year":"1984","unstructured":"Anderson, J. 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