{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:07:00Z","timestamp":1784606820651,"version":"3.55.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Department of Education and Training | Australian Research Council","doi-asserted-by":"publisher","award":["LP140100315"],"award-info":[{"award-number":["LP140100315"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Chlamydia pecorum<\/jats:named-content>\n            is an important intracellular bacterium that causes a range of diseases in animals, including a native Australian marsupial, the koala. In humans and animals, a gamma interferon (IFN-\u03b3)-mediated immune response is important for the control of intracellular bacteria. The present study tested the hypotheses that\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            can escape IFN-\u03b3-mediated depletion of host cell tryptophan pools. In doing so, we demonstrated that, unlike\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            is completely resistant to IFN-\u03b3 in human epithelial cells. While the growth of\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            was inhibited in tryptophan-deficient medium, it could be restored by the addition of kynurenine, anthranilic acid, and indole, metabolites that could be exploited by the gene products of the\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            tryptophan biosynthesis operon. We also found that expression of\n            <jats:italic>trp<\/jats:italic>\n            genes was detectable only when\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            was grown in tryptophan-free medium, with gene repression occurring in response to the addition of kynurenine, anthranilic acid, and indole. When grown in bovine kidney epithelial cells, bovine IFN-\u03b3 also failed to restrict the growth of\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            , while\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            was inhibited, suggesting that\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            could use the same mechanisms to evade the immune response\n            <jats:italic>in vivo<\/jats:italic>\n            in its natural host. Highlighting the different mechanisms triggered by IFN-\u03b3, however, both species failed to grow in murine McCoy cells treated with murine IFN-\u03b3. This work confirms previous hypotheses about the potential survival of\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            after IFN-\u03b3-mediated host cell tryptophan depletion and raises questions about the immune pathways used by the natural hosts of\n            <jats:named-content content-type=\"genus-species\">C. pecorum<\/jats:named-content>\n            to control the widespread pathogen.\n          <\/jats:p>","DOI":"10.1128\/iai.00714-17","type":"journal-article","created":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T11:22:47Z","timestamp":1516706567000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Characterization of the\n            <i>In Vitro<\/i>\n            Chlamydia pecorum Response to Gamma Interferon"],"prefix":"10.1128","volume":"86","author":[{"given":"M. Mominul","family":"Islam","sequence":"first","affiliation":[{"name":"Centre for Animal Health Innovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-2562","authenticated-orcid":false,"given":"Martina","family":"Jelocnik","sequence":"additional","affiliation":[{"name":"Centre for Animal Health Innovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelmina M.","family":"Huston","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Timms","sequence":"additional","affiliation":[{"name":"Centre for Animal Health Innovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Polkinghorne","sequence":"additional","affiliation":[{"name":"Centre for Animal Health Innovation, University of the Sunshine Coast, Sippy Downs, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(97)01061-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.1.143-190.1991"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00097"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.1843-1855.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/652394"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00027"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.3998"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)90356-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01045-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-190"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6979-6987.2000"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.225-238.2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504198102"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90542-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203937200"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-9-research0051"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01660-05"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.10.6038-6040.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17993"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5423-5429.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7927-7932.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12701"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg321"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04029.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00106-15"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-7-29"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.02.026"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.09.022"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1726-1729.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.09.003"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-016-0832-3"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044961"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.01.004"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-667"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-2053-8"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-23"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00180"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3832917"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7063-7072.2004"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-016-0903-4"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.11.1907934"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00310-09"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05204-11"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00959.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00368-3"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/7716"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2007.09.026"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.749"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00992-13"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.035"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12052"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01244-13"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.03.091"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156094"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1086\/432070"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01417-16"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515966112"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.06.039"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.10.5909"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172738"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-77"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00714-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00714-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:49:00Z","timestamp":1646477340000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00714-17"}},"subtitle":[],"editor":[{"given":"Vincent B.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":62,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1128\/IAI.00714-17"],"URL":"https:\/\/doi.org\/10.1128\/iai.00714-17","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"2017-10-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00714-17"}}