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During its commensal and pathogenic lifestyles,\n            <jats:italic>S. agalactiae<\/jats:italic>\n            colonizes and invades a number of host compartments, thereby interacting with different host proteins. In the present study, the serine-rich repeat protein Srr-1 from\n            <jats:italic>S. agalactiae<\/jats:italic>\n            was functionally investigated. Immunofluorescence microscopy showed that Srr-1 was localized on the surface of streptococcal cells. The Srr-1 protein was shown to interact with a 62-kDa protein in human saliva, which was identified by matrix-assisted laser desorption ionization-time-of-flight analysis as human keratin 4 (K4). Immunoblot and enzyme-linked immunosorbent assay experiments allowed us to narrow down the K4 binding domain in Srr-1 to a region of 157 amino acids (aa). Furthermore, the Srr-1 binding domain of K4 was identified in the C-terminal 255 aa of human K4. Deletion of the\n            <jats:italic>srr-1<\/jats:italic>\n            gene in the genome of\n            <jats:italic>S. agalactiae<\/jats:italic>\n            revealed that this gene plays a role in bacterial binding to human K4 and that it is involved in adherence to epithelial HEp-2 cells. Binding to immobilized K4 and adherence to HEp-2 cells were restored by introducing the\n            <jats:italic>srr-1<\/jats:italic>\n            gene on a shuttle plasmid into the\n            <jats:italic>srr-1<\/jats:italic>\n            mutant. Furthermore, incubation of HEp-2 cells with the K4 binding domain of Srr-1 blocked\n            <jats:italic>S. agalactiae<\/jats:italic>\n            adherence to epithelial cells in a dose-dependent fashion. This is the first report describing the interaction of a bacterial protein with human K4.\n          <\/jats:p>","DOI":"10.1128\/iai.00717-07","type":"journal-article","created":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T00:58:49Z","timestamp":1187657929000},"page":"5405-5414","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["The Surface Protein Srr-1 of\n            <i>Streptococcus agalactiae<\/i>\n            Binds Human Keratin 4 and Promotes Adherence to Epithelial HEp-2 Cells"],"prefix":"10.1128","volume":"75","author":[{"given":"Ulrike","family":"Samen","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard J.","family":"Eikmanns","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter J.","family":"Reinscheid","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"},{"name":"University of Applied Sciences Bonn-Rhein-Sieg, 53359 Rheinbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Borges","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112072200"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610940200"},{"key":"e_1_3_2_6_2","first-page":"154","year":"2000","unstructured":"Balter, S., C. 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