{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:11:37Z","timestamp":1779351097687,"version":"3.51.4"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n            , an important human intestinal pathogen, is responsible for the diarrheal disease cholera. The pathogenesis of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            is a highly coordinated process that involves diverse regulatory factors. It has recently been demonstrated that disruption of the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            <jats:italic>fadD<\/jats:italic>\n            gene, encoding a long-chain fatty acyl coenzyme A (acyl-CoA) ligase, drastically reduces expression of the major virulence genes and\n            <jats:italic>in vivo<\/jats:italic>\n            lethality of this important human pathogen. This effect was due to reduced membrane localization of the central virulence regulator TcpP. In this study, the reason for the impaired membrane localization of TcpP in the\n            <jats:italic>fadD<\/jats:italic>\n            mutant was investigated. We demonstrate that extracytoplasmic stress is induced in the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            \u0394\n            <jats:italic>fadD<\/jats:italic>\n            strain. In response to the extracytoplasmic stress, the integral membrane protease RseP is activated and degrades the membrane-localized TcpP in the\n            <jats:italic>fadD<\/jats:italic>\n            mutant strain. Indeed, disruption of the\n            <jats:italic>rseP<\/jats:italic>\n            gene in a\n            <jats:italic>fadD<\/jats:italic>\n            mutant background restored membrane localization of TcpP and expression of the downstream virulence genes\n            <jats:italic>toxT<\/jats:italic>\n            ,\n            <jats:italic>ctxA<\/jats:italic>\n            , and\n            <jats:italic>tcpA<\/jats:italic>\n            . Increased expression of the \u03c3\n            <jats:sup>E<\/jats:sup>\n            regulon genes in ethanol-treated wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            indicated that ethanol exposure could induce an extracytoplasmic stress response in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            . Ethanol treatment also led to activation of the RseP protease activity and resulted in degradation of membrane-localized TcpP and subsequent reduction in expression of the virulence genes. Taken together, these results suggest that extracytoplasmic stress response\n            <jats:italic>per se<\/jats:italic>\n            reduces virulence of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">V. cholerae<\/jats:named-content>\n            by impairing membrane localization of TcpP.\n          <\/jats:p>","DOI":"10.1128\/iai.00722-13","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T05:35:49Z","timestamp":1375767349000},"page":"3935-3941","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Reduced Virulence of the Vibrio cholerae\n            <i>fadD<\/i>\n            Mutant Is Due to Induction of the Extracytoplasmic Stress Response"],"prefix":"10.1128","volume":"81","author":[{"given":"Epshita","family":"Chatterjee","sequence":"first","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rukhsana","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Cholera fact sheet","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. 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