{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:48:52Z","timestamp":1790729332012,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Streptococcus pneumoniae<\/jats:named-content>\n            (pneumococcus) frequently colonizes the human nasopharynx and is an important cause of pneumonia, meningitis, sinusitis, and otitis media. The outer cell surface of pneumococcus may assume various degrees of negative charge depending on the polysaccharide capsule, of which more than 90 serotypes have been identified. The negative charge of capsular polysaccharides has been proposed to electrostatically repel pneumococci from phagocytic cells, and avoidance of phagocytosis correlates with higher carriage prevalence. We hypothesized that the surface charge of pneumococcus contributes to its success in nasopharyngeal carriage by modulating resistance to phagocyte-mediated killing. Here, we measured the surface charge (zeta potential) of laboratory-constructed strains that share a genetic background but differ in serotype and of clinical strains that differ in serotype and genetic background. A more negative surface charge correlated with higher resistance to nonopsonic killing by human neutrophils\n            <jats:italic>in vitro<\/jats:italic>\n            . In addition, a more negative zeta potential was associated with higher carriage prevalence in human populations before and after the widespread use of the pneumococcal conjugate vaccine PCV7. We also confirmed that capsule is the major determinant of net surface charge in clinical isolates with diverse backgrounds. We noted that exceptions exist to the idea that a higher magnitude of negative charge predicts higher prevalence. The results indicated that zeta potential is strongly influenced by pneumococcal capsule type but is unlikely to be the only important mechanism by which capsule interacts with host.\n          <\/jats:p>","DOI":"10.1128\/iai.00724-13","type":"journal-article","created":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T05:24:33Z","timestamp":1380605073000},"page":"4519-4524","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Surface Charge of Streptococcus pneumoniae Predicts Serotype Distribution"],"prefix":"10.1128","volume":"81","author":[{"given":"Yuan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Department of Immunology &amp; Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel M.","family":"Weinberger","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Department of Immunology &amp; Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"},{"name":"Department of Epidemiology of Microbial Diseases, Yale School of Public Health and School of Medicine, New Haven, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudette M.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Department of Immunology &amp; Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Trzci\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Department of Immunology &amp; Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"},{"name":"Department of Pediatric Immunology and Infectious Diseases, UMC Utrecht-WKZ, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Lipsitch","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Department of Immunology &amp; Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.53"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70083-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/319750"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/313608"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/374624"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/423820"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.431-435.2005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/3.Supplement_1.S1"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm839"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00862-12"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.epidem.2010.03.005"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62225-8"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022823"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/521626"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2004-2338"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000154326.77617.3e"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.297.16.1784"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.68"},{"key":"e_1_3_3_21_2","first-page":"85","volume-title":"Streptococcus pneumoniae: molecular biology & mechanisms of disease","author":"Kamerling J","year":"2000","unstructured":"KamerlingJ. 2000. Pneumococcal polysaccharides: a chemical view, p 85\u201393. In TomaszA (ed), Streptococcus pneumoniae: molecular biology & mechanisms of disease. 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