{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:24:00Z","timestamp":1792185840487,"version":"4.4.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several bacterial pathogens persist and survive in the host by modulating host cell death pathways. We previously demonstrated that\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            , a Gram-negative pathogen responsible for the sexually transmitted infection gonorrhea, protects against exogenous induction of apoptosis in human cervical epithelial cells. However, induction of cell death by\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            has also been reported in other cell types. The mechanisms by which\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            modulates cell death are not clear, although a role for the inhibitor of apoptosis-2 (cIAP2) has been proposed. In this study, we confirmed that\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            induces production of cIAP2 in human cervical epithelial cells. High levels of intracellular cIAP2 were detected early after\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            stimulation, which was followed by a marked decrease at 24 h. At this time point, we observed increased levels of extracellular cIAP2 associated with exosomes and an overall increase in production of exosomes. Inhibition of cIAP2 in\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -stimulated epithelial cells resulted in increased cell death and interleukin-1\u03b2 (IL-1\u03b2) production. Collectively these results indicate that\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            stimulation of human endocervical epithelial cells induces the release of cIAP2, an essential regulator of cell death and immune signaling.\n          <\/jats:p>","DOI":"10.1128\/iai.00732-15","type":"journal-article","created":{"date-parts":[[2015,6,16]],"date-time":"2015-06-16T02:06:42Z","timestamp":1434420402000},"page":"3410-3417","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2"],"prefix":"10.1128","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5483-0515","authenticated-orcid":false,"given":"Kathleen","family":"Nudel","sequence":"first","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Massari","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline A.","family":"Genco","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, Massachusetts, USA"},{"name":"Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201004096"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3720"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2421"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.06.007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201108081"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2014-0164"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5354-5363.2000"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(02)00019-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.06.006"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5840-5848.2001"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.2119\/2008-00035.Gribar"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2013.109"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1356-7"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/rr25"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050004"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.2.339"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00300.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00012-06"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00153-08"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802696"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01366-08"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.8.2215"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510863200"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2889"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711122105"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.011"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.138"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020114"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.12.003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.4.847"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00996-09"},{"key":"e_1_3_3_33_2","first-page":"Unit 3.22","article-title":"Isolation and characterization of exosomes from cell culture supernatants and biological fluids","volume":"3","author":"Th\u00e9ry C","year":"2006","unstructured":"Th\u00e9ry C, Amigorena S, Raposo G, Clayton A. 2006. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol Chapter 3:Unit 3.22.","journal-title":"Curr Protoc Cell Biol"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.12.2505"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-010-0534-4"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201211138"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.030"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.06.011"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.59"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.06.006"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306541200"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.07.136"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1621"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2012.00038"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2005.05.011"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28164"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200562"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.2.699-708.2006"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.03.036"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.01.012"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.02.003"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00732-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00732-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:08:50Z","timestamp":1646474930000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00732-15"}},"subtitle":[],"editor":[{"given":"R. P.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":50,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1128\/IAI.00732-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.00732-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}