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Negative-control groups were immunized i.m. and s.c. with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Neisseria gonorrhoeae<\/jats:named-content>recombinant porin B (<jats:italic>Ng<\/jats:italic>-rPorB) or i.n. with Eagle's minimal essential medium (MEM-0). Following vaccination, the mice developed antibodies not only against the homologous serovar but also against heterologous serovars. The rMOMP-vaccinated animals also mounted cell-mediated immune responses as assessed by a lymphoproliferative assay. Four weeks after the last immunization, mice were challenged i.n. with 10<jats:sup>4<\/jats:sup>inclusion-forming units (IFU) of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. muridarum<\/jats:named-content>. The mice were weighed for 10 days and euthanized, and the number of IFU in their lungs was determined. At 10 days postinfection (p.i.), mice immunized with the rMOMP of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. muridarum<\/jats:named-content>or<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. trachomatis<\/jats:named-content>D, E, or F had lost 4%, 6%, 8%, and 8% of their initial body weight, respectively, significantly different from the negative-control groups (<jats:italic>Ng<\/jats:italic>-rPorB, 13%; MEM-0, 19%;<jats:italic>P<\/jats:italic>&lt; 0.05). The median number of IFU recovered from the lungs of mice immunized with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. muridarum<\/jats:named-content>rMOMP was 0.13 \u00d7 10<jats:sup>6<\/jats:sup>. The median number of IFU recovered from mice immunized with rMOMP from serovars D, E, and F were 0.38 \u00d7 10<jats:sup>6<\/jats:sup>, 7.56 \u00d7 10<jats:sup>6<\/jats:sup>, and 11.94 \u00d7 10<jats:sup>6<\/jats:sup>IFU, respectively. All the rMOMP-immunized animals had significantly less IFU than the<jats:italic>Ng<\/jats:italic>-rPorB (40 \u00d7 10<jats:sup>6<\/jats:sup>)- or MEM-0 (70 \u00d7 10<jats:sup>6<\/jats:sup>)-immunized mice (<jats:italic>P<\/jats:italic>&lt; 0.05). In conclusion, vaccination with rMOMP can elicit protection against homologous and heterologous<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Chlamydia<\/jats:named-content>serovars.<\/jats:p>","DOI":"10.1128\/iai.00734-12","type":"journal-article","created":{"date-parts":[[2013,3,12]],"date-time":"2013-03-12T00:53:09Z","timestamp":1363049589000},"page":"1741-1750","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Vaccination with the Recombinant Major Outer Membrane Protein Elicits Antibodies to the Constant Domains and Induces Cross-Serovar Protection against Intranasal Challenge with Chlamydia trachomatis"],"prefix":"10.1128","volume":"81","author":[{"given":"Delia F.","family":"Tifrea","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, University of California, Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pooja","family":"Ralli-Jain","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, University of California, Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukumar","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, University of California, Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"de la Maza","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, University of California, Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Human chlamydial infections.","author":"Schachter J","year":"1978","unstructured":"SchachterJDawsonC. 1978. 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