{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:22:04Z","timestamp":1789939324648,"version":"4.0.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            is the causative agent of melioidosis and represents\na potential bioterrorism threat. In the current studies we have examined gene expression in\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            in an animal model of acute melioidosis using whole-genome microarrays. Gene expression\nprofiles were generated by comparing transcriptional levels of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            -expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes\nof infection to those from bacteria grown in vitro. Differentially expressed genes were similar in infected livers irrespective of the route of infection. Reduced expression of a number of housekeeping genes suggested a lower bacterial growth rate during infection. Energy production during growth in vivo involved specific biochemical pathways such as isomerization of 3-phosphoglycerate, catabolism of\n            <jats:sc>d<\/jats:sc>\n            -glucosamine and inositol, and biosynthesis of particular amino acids. In addition, the induction of genes known to be involved in oxidative phosphorylation including ubiquinol oxidase, ferredoxin oxidoreductase, and formate dehydrogenase enzymes suggested the use of alternative pathways for energy production, while the expression of genes coding for ATP-synthase and NADH-dehydrogenase enzymes was reduced. Our studies have identified differentially expressed genes which include potential virulence genes such as those for a putative phospholipase C and a putative two-component regulatory system, and they have also provided a better understanding of bacterial metabolism in response to the host environment during acute melioidosis.\n          <\/jats:p>","DOI":"10.1128\/iai.00737-06","type":"journal-article","created":{"date-parts":[[2006,9,20]],"date-time":"2006-09-20T23:06:35Z","timestamp":1158793595000},"page":"5465-5476","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Genome-Wide Expression Analysis of\n            <i>Burkholderia pseudomallei<\/i>\n            Infection ina Hamster Model of Acute Melioidosis"],"prefix":"10.1128","volume":"74","author":[{"given":"Apichai","family":"Tuanyok","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Faculty of Medicine,\rUniversity of Calgary Health Sciences Centre, 3330 Hospital Drive, NW, Calgary, Alberta, Canada T2N\r4N1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Tom","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Faculty of Medicine,\rUniversity of Calgary Health Sciences Centre, 3330 Hospital Drive, NW, Calgary, Alberta, Canada T2N\r4N1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Dunbar","sequence":"additional","affiliation":[{"name":"Biosciences Division, Los Alamos National Laboratory, Los Alamos, New\rMexico 87545"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald E.","family":"Woods","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Faculty of Medicine,\rUniversity of Calgary Health Sciences Centre, 3330 Hospital Drive, NW, Calgary, Alberta, Canada T2N\r4N1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2005.tb06739.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1104.041051"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026889600739X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.5.890"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.08.069"},{"key":"e_1_3_2_7_2","first-page":"272","volume":"27","year":"2003","unstructured":"Cheng, A. 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