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Building on our previous experiments using a single female pig-tailed macaque as a model for\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    infection (G. E. Wood, S. L. Iverson-Cabral, D. L. Patton, P. K. Cummings, Y. T. Cosgrove Sweeney, and P. A. Totten, Infect Immun 81:2938\u20132951, 2013,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/IAI.01322-12\">https:\/\/doi.org\/10.1128\/IAI.01322-12<\/jats:ext-link>\n                    ), we cervically inoculated eight additional animals, two of which were simultaneously inoculated in salpingeal tissue autotransplanted into abdominal pockets. Viable\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    persisted in the lower genital tract for 8 weeks in three animals, 4 weeks in two, and 1 week in one; two primates resisted infection. In both animals inoculated in salpingeal pockets, viable\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    was recovered for 2 weeks. Recovery of viable\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    from lower genital tract specimens was improved by diluting the specimen in broth and by Vero cell coculture. Ascension to upper reproductive tract tissues was not detected, even among three persistently infected animals.\n                    <jats:named-content content-type=\"genus-species\">M. genitalium-<\/jats:named-content>\n                    specific serum antibodies targeting the immunodominant MgpB and MgpC proteins appeared within 1 week in three animals inoculated both cervically and in salpingeal pockets and in one of three persistently infected animals inoculated only in the cervix.\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    -specific IgG, but not IgA, was detected in cervical secretions of serum antibody-positive animals, predominantly against MgpB and MgpC, but was insufficient to clear\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    lower tract infection. Our findings further support female pig-tailed macaques as a model of\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    infection, persistence, and immune evasion.\n                  <\/jats:p>","DOI":"10.1128\/iai.00738-16","type":"journal-article","created":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T22:10:23Z","timestamp":1479766223000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Experimental Infection of Pig-Tailed Macaques (Macaca nemestrina) with Mycoplasma genitalium"],"prefix":"10.1128","volume":"85","author":[{"given":"Gwendolyn E.","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy L.","family":"Patton","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter K.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie L.","family":"Iverson-Cabral","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Totten","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA"},{"name":"Department of Global Health, Pathobiology Interdisciplinary Program, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3854-3856.2002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir702"},{"key":"e_1_3_2_4_2","first-page":"265","article-title":"Mycoplasma genitalium: a cause of male urethritis?","volume":"69","author":"Jensen JS","year":"1993","unstructured":"Jensen JS Orsum R Dohn B Uldum S Worm AM Lind K . 1993. 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