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Salmonellae establish infection and avoid clearance by the immune system by mechanisms that are not well understood. We previously showed that\n            <jats:sc>l<\/jats:sc>\n            -asparaginase II produced by\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium (\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium) inhibits T cell responses and mediates virulence. In addition, we previously showed that asparagine deprivation such as that mediated by\n            <jats:sc>l<\/jats:sc>\n            -asparaginase II of\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium causes suppression of activation-induced T cell metabolic reprogramming. Here, we report that\n            <jats:italic>STM3997<\/jats:italic>\n            , which encodes a homolog of disulfide bond protein A (\n            <jats:italic>dsbA<\/jats:italic>\n            ) of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , is required for\n            <jats:sc>l<\/jats:sc>\n            -asparaginase II stability and function. Furthermore, we report that\n            <jats:sc>l<\/jats:sc>\n            -asparaginase II localizes primarily to the periplasm and acts together with\n            <jats:sc>l<\/jats:sc>\n            -asparaginase I to provide\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium the ability to catabolize asparagine and assimilate nitrogen. Importantly, we determined that, in a murine model of infection,\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium lacking both\n            <jats:sc>l<\/jats:sc>\n            -asparaginase I and II genes competes poorly with wild-type\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium for colonization of target tissues. Collectively, these results indicate that asparagine catabolism contributes to\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium virulence, providing new insights into the competition for nutrients at the host-pathogen interface.\n          <\/jats:p>","DOI":"10.1128\/iai.00740-16","type":"journal-article","created":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T02:50:31Z","timestamp":1479178231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Contribution of Asparagine Catabolism to Salmonella Virulence"],"prefix":"10.1128","volume":"85","author":[{"given":"Patrick A.","family":"McLaughlin","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology and Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McClelland","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee-Jeong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, Texas A&amp;M University System Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Porwollik","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Bogomolnaya","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, Texas A&amp;M University System Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juei-Suei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology and Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helene","family":"Andrews-Polymenis","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, Texas A&amp;M University System Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrianus W. 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