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We previously identified heat shock protein 60 (HSP60) as a target of the humoral response in paracoccidioidomycosis. Herein we expressed the gene encoding HSP60 in<jats:italic>Escherichia coli<\/jats:italic>and analyzed the immunological activity of this recombinant antigen. The immunization of BALB\/c mice with recombinant protein emulsified in adjuvant stimulated a cellular immune response. Splenocytes from immunized mice proliferated in response to antigen and released interleukin-12 and gamma interferon (IFN-\u03b3). Vaccination with HSP60 reduced the fungal burden in mice given 10<jats:sup>6<\/jats:sup>or 10<jats:sup>7<\/jats:sup>yeasts and protected mice from a lethal challenge. The efficacy of the vaccination was blunted by the neutralization of IFN-\u03b3. CD4<jats:sup>+<\/jats:sup>cells were necessary for the efficacy of the vaccination in both the afferent and efferent phases. Thus, we have demonstrated that this immunodominant antigen is a candidate for the development of a vaccine against this fungus.<\/jats:p>","DOI":"10.1128\/iai.00753-07","type":"journal-article","created":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T00:34:43Z","timestamp":1216082083000},"page":"4214-4221","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Vaccination with Heat Shock Protein 60 Induces a Protective Immune Response against Experimental<i>Paracoccidioides brasiliensis<\/i>Pulmonary Infection"],"prefix":"10.1128","volume":"76","author":[{"given":"Renata","family":"de Bastos Ascenc\u0327o Soares","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Biologia Molecular, ICBII, Universidade Federal de Goia\u0301s, 74001-970 Goia\u0302nia, Goia\u0301s, Brazil"},{"name":"Veterans Affairs Hospital, Division of Infectious Diseases, Department of Medicine, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267-0560"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Gomez","sequence":"additional","affiliation":[{"name":"Veterans Affairs Hospital, Division of Infectious Diseases, Department of Medicine, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267-0560"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301lia Maria","family":"de Almeida Soares","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biologia Molecular, ICBII, Universidade Federal de Goia\u0301s, 74001-970 Goia\u0302nia, Goia\u0301s, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"George S.","family":"Deepe","sequence":"additional","affiliation":[{"name":"Veterans Affairs Hospital, Division of Infectious Diseases, Department of Medicine, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267-0560"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.4049\/jimmunol.152.7.3656","volume":"152","year":"1994","unstructured":"Anderton, S. 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