{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:53:15Z","timestamp":1791060795226,"version":"4.1.0"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>spp. are facultative intracellular bacterial pathogens responsible for brucellosis, a worldwide zoonosis that causes abortion in domestic animals and chronic febrile disease associated with serious complications in humans. There is currently no approved vaccine against human brucellosis, and antibiotic therapy is long and costly. Development of a safe protective vaccine requires a better understanding of the roles played by components of adaptive immunity in the control of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>infection. The importance of lymphocyte subsets in the control of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella<\/jats:named-content>growth has been investigated separately by various research groups and remains unclear or controversial. Here, we used a large panel of genetically deficient mice to compare the importance of B cells, transporter associated with antigen processing (TAP-1), and major histocompatibility complex class II-dependent pathways of antigen presentation as well as T helper 1 (Th1), Th2, and Th17-mediated responses on the immune control of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Brucella melitensis<\/jats:named-content>16 M infection. We clearly confirmed the key function played by gamma interferon (IFN-\u03b3)-producing Th1 CD4<jats:sup>+<\/jats:sup>T cells in the control of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>infection, whereas IFN-\u03b3-producing CD8<jats:sup>+<\/jats:sup>T cells or B cell-mediated humoral immunity plays only a modest role in the clearance of bacteria during primary infection. In the presence of a Th1 response, Th2 or Th17 responses do not really develop or play a positive or negative role during the course of<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. melitensis<\/jats:named-content>infection. On the whole, these results could improve our ability to develop protective vaccines or therapeutic treatments against brucellosis.<\/jats:p>","DOI":"10.1128\/iai.00761-12","type":"journal-article","created":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T10:25:58Z","timestamp":1348568758000},"page":"4271-4280","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Crucial Role of Gamma Interferon-Producing CD4<sup>+<\/sup>Th1 Cells but Dispensable Function of CD8<sup>+<\/sup>T Cell, B Cell, Th2, and Th17 Responses in the Control of Brucella melitensis Infection in Mice"],"prefix":"10.1128","volume":"80","author":[{"given":"Marie-Alice","family":"Vitry","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, NARILIS, Facult\u00e9s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"De Trez","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislas","family":"Goriely","sequence":"additional","affiliation":[{"name":"Institut d'Immunologie M\u00e9dicale, Universit\u00e9 Libre de Bruxelles, Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Dumoutier","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Brussels Branch, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuo","family":"Akira","sequence":"additional","affiliation":[{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University Yamadaoka, Suita City, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Ryffel","sequence":"additional","affiliation":[{"name":"INEM-UMR7355, Molecular Immunology, Institute Transgenose, University and CNRS Orleans, and IIDMM, University of Cape Town, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Carlier","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Facult\u00e9 de M\u00e9decine, and Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, NARILIS, Facult\u00e9s Universitaires Notre-Dame de la Paix, Namur, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Muraille","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Facult\u00e9 de M\u00e9decine, and Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.4049\/jimmunol.143.10.3330","article-title":"Temporal development of protective cell-mediated and humoral immunity in BALB\/c mice infected with Brucella abortus","volume":"143","author":"Araya LN","year":"1989","unstructured":"ArayaLNElzerPHRoweGEEnrightFMWinterAJ. 1989. Temporal development of protective cell-mediated and humoral immunity in BALB\/c mice infected with Brucella abortus. J. Immunol. 143:3330\u20133337.","journal-title":"J. Immunol."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05321-11"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v26.i5.30"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00222-5"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.07.020"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/589494"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.4049\/jimmunol.162.5.2761","article-title":"Dissecting NK cell development using a novel alymphoid mouse model: investigating the role of the c-abl proto-oncogene in murine NK cell differentiation","volume":"162","author":"Colucci F","year":"1999","unstructured":"ColucciF. 1999. Dissecting NK cell development using a novel alymphoid mouse model: investigating the role of the c-abl proto-oncogene in murine NK cell differentiation. J. Immunol. 162:2761\u20132765.","journal-title":"J. Immunol."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.1.309"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.3.1322"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.8.5182"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002575"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90448-8"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03017.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02622.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.4.2004"},{"key":"e_1_3_3_18_2","first-page":"651","article-title":"Antibody-mediated protection against Brucella abortus in BALB\/c mice at successive periods after infection: variation between virulent strain 2308 and attenuated vaccine strain 19","volume":"82","author":"Elzer PH","year":"1994","unstructured":"ElzerPH. 1994. Antibody-mediated protection against Brucella abortus in BALB\/c mice at successive periods after infection: variation between virulent strain 2308 and attenuated vaccine strain 19. Immunology 82:651\u2013658.","journal-title":"Immunology"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.3.1130-1133.1995"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1996.tb00136.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.058"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.05.029"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3061"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq171"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-29"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050193"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni0809-806"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/422329"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5314-5320.2000"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90095-7"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80086-2"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085458"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1449"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.788"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3181\/0710-RM-270"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/350423a0"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2433"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.12.8098"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1688"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.3.1887"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2494(91)90007-6"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0769-2625(87)80030-2"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(05)80017-9"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80413-6"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04754"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00982.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260722"},{"key":"e_1_3_3_49_2","first-page":"99","article-title":"T cell subsets in granulomatous inflammation and immunity to L. Monocytogenes and B. abortus","author":"Mielke ME","year":"1991","unstructured":"MielkeME. 1991. T cell subsets in granulomatous inflammation and immunity to L. Monocytogenes and B. abortus. Behring Inst. Mitt., p 99\u2013111.","journal-title":"Behring Inst. Mitt."},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90030-G"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.3.961-963.1986"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2001.tb00536.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01258.x"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00391-6"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.11.110"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250922"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016203"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(82)90502-0"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00211.x"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01896-06"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01114-08"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2006.10.003"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.11.7891"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7688139"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021978"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.10.4407-4409.1992"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20120504"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.6.3786"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.06.007"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80068-6"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.5137-5143.2005"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3438-3444.1989"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.3.4424"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.11.4899-4901.1993"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00761-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00761-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T23:02:25Z","timestamp":1687734145000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00761-12"}},"subtitle":[],"editor":[{"given":"R. P.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":74,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1128\/IAI.00761-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.00761-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"2012-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}