{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:38:37Z","timestamp":1790919517028,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            lethal toxin (LT) was characterized in plasma from infected African Green monkeys, rabbits, and guinea pigs. In all cases, during the terminal phase of infection only the protease-activated 63-kDa form of protective antigen (PA\n            <jats:sub>63<\/jats:sub>\n            ) and the residual 20-kDa fragment (PA\n            <jats:sub>20<\/jats:sub>\n            ) were detected in the plasma. No uncut PA with a molecular mass of 83 kDa was detected in plasma from toxemic animals during the terminal stage of infection. PA\n            <jats:sub>63<\/jats:sub>\n            was largely associated with lethal factor (LF), forming LT. Characterization of LT by Western blotting, capture enzyme-linked immunosorbent assay, and size exclusion chromatography revealed that the antiphagocytic poly-\u03b3-\n            <jats:sc>d<\/jats:sc>\n            -glutamic acid (\u03b3-DPGA) capsule released from\n            <jats:italic>B. anthracis<\/jats:italic>\n            bacilli was associated with LT in animal blood in variable amounts. While the nature of this in vivo association is not understood, we were able to determine that a portion of these LT\/\u03b3-DPGA complexes retained LF protease activity. Our findings suggest that the in vivo LT complexes differ from in vitro-produced LT and that including \u03b3-DPGA when examining the effects of LT on specific immune cells in vitro may reveal novel and important roles for \u03b3-DPGA in anthrax pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/iai.00764-08","type":"journal-article","created":{"date-parts":[[2008,12,9]],"date-time":"2008-12-09T01:25:40Z","timestamp":1228785940000},"page":"749-755","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Association of\n            <i>Bacillus anthracis<\/i>\n            Capsule with Lethal Toxin during Experimental Infection"],"prefix":"10.1128","volume":"77","author":[{"given":"J. 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