{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:31:30Z","timestamp":1791228690871,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>P.69 pertactin (P.69 Prn), an adhesion molecule from the causative agent of pertussis,<jats:italic>Bordetella pertussis<\/jats:italic>, is present in cellular and most acellular vaccines that are currently used worldwide. Although both humoral immunity and cellular immunity directed against P.69 Prn have been implicated in protective immune mechanisms, the identities of CD4<jats:sup>+<\/jats:sup>T-cell epitopes on the P.69 Prn protein remain unknown. Here, a single I-A<jats:sup>d<\/jats:sup>-restricted<jats:italic>B. pertussis<\/jats:italic>conserved CD4<jats:sup>+<\/jats:sup>T-cell epitope at the N terminus of P.69 Prn was identified by using a BALB\/c T-cell hybridoma. The epitope appeared immunodominant among four other minor strain-conserved P.69 Prn epitopes recognized after vaccination and<jats:italic>B. pertussis<\/jats:italic>infection, and it was capable of evoking a Th1\/Th17-type cytokine response.<jats:italic>B. pertussis<\/jats:italic>P.69 Prn immune splenocytes did not cross-react with natural variants of the epitope as present in<jats:italic>Bordetella parapertussis<\/jats:italic>and<jats:italic>Bordetella bronchiseptica<\/jats:italic>. Finally, it was found that the immunodominant P.69 Prn epitope is broadly recognized in the human population by CD4<jats:sup>+<\/jats:sup>T cells in an HLA-DQ-restricted manner. During<jats:italic>B. pertussis<\/jats:italic>infection, the epitope was associated with a Th1-type CD4<jats:sup>+<\/jats:sup>T-cell response. Hence, this novel P.69 Prn epitope is involved in CD4<jats:sup>+<\/jats:sup>T-cell immunity after<jats:italic>B. pertussis<\/jats:italic>vaccination and infection in mice and, more importantly, in humans. Thus, it may provide a useful tool for the evaluation of the type, magnitude, and maintenance of<jats:italic>B. pertussis<\/jats:italic>-specific CD4<jats:sup>+<\/jats:sup>T-cell mechanisms in preclinical and clinical vaccine studies.<\/jats:p>","DOI":"10.1128\/iai.00769-08","type":"journal-article","created":{"date-parts":[[2008,11,18]],"date-time":"2008-11-18T01:55:54Z","timestamp":1226973354000},"page":"896-903","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Immunodominance in Mouse and Human CD4<sup>+<\/sup>T-Cell Responses Specific for the<i>Bordetella pertussis<\/i>Virulence Factor P.69 Pertactin"],"prefix":"10.1128","volume":"77","author":[{"given":"Rachel M.","family":"Stenger","sequence":"first","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martien C. M.","family":"Poelen","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ed E.","family":"Moret","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betsy","family":"Kuipers","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven C. M.","family":"Bruijns","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hoogerhout","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Hijnen","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Preventable Diseases, National Institute for Public Health and the Environment, Bilthoven"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey J.","family":"King","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Preventable Diseases, National Institute for Public Health and the Environment, Bilthoven"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frits R.","family":"Mooi","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Preventable Diseases, National Institute for Public Health and the Environment, Bilthoven"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire J. P.","family":"Boog","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301cile A. C. M.","family":"van Els","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Research, Netherlands Vaccine Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"955","year":"1988","unstructured":"Ad Hoc Group for the Study of Pertussis Vaccines. 1988. Placebo-controlled trial of two acellular pertussis vaccines in Sweden\u2014protective efficacy and adverse events. Lanceti:955-960.","journal-title":"Lancet"},{"key":"e_1_3_2_3_2","first-page":"145","volume":"21","year":"1997","unstructured":"Andrews, R., A. Herceg, and C. Roberts. 1997. Pertussis notifications in Australia, 1991 to 1997. Commun. Dis. Intell.21:145-148.","journal-title":"Commun. Dis. Intell."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.6.2168-2174.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra020100"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199405000-00002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2003.07.004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00226-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68848-X"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970211"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.06.021"},{"key":"e_1_3_2_12_2","first-page":"45","volume":"21","year":"1995","unstructured":"De Serres, G., N. Boulianne, F. M. Douville, and B. Duval. 1995. Pertussis in Quebec: ongoing epidemic since the late 1980s. Can. Commun. Dis. Rep.21:45-48.","journal-title":"Can. Commun. Dis. Rep."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/381090a0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(98)70395-6"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.4.5.757"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/514776"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199602083340602"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyh012"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00356-5"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.3.329"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7980"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540701642138"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.01.014"},{"key":"e_1_3_2_24_2","first-page":"49","volume":"27","year":"1954","unstructured":"Kasuga, T., Y. Nakase, K. Ukishima, and K. Takatsu. 1954. Studies on Haemophilus pertussis. IV. Preventive potency of each phase organisms of H. pertussis in mice. Kitasato Arch. Exp. Med.27:49-55.","journal-title":"Kitasato Arch. Exp. Med."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.2.486-490.1993"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-11-2885"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.11.1841"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1086\/315527"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.06.046"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/biol.1999.0184"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01421-6"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.2.399-410.1993"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.594-602.1998"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7317-7323.2005"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050455"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0707.017708"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.2.670-675.1998"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199705000-00011"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.8.3190-3198.1993"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1086\/593682"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00401.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.04.018"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.6.1957"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90318-5"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00227-8"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25350"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(99)00155-8"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5364-5367.2004"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.4.1459"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000160914.59160.41"},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.4049\/jimmunol.143.6.1822","volume":"143","year":"1989","unstructured":"White, J., M. Blackman, J. Bill, J. Kappler, P. Marrack, D. P. Gold, and W. Born. 1989. Two better cell lines for making hybridomas expressing specific T cell receptors. J. Immunol.143:1822-1825.","journal-title":"J. Immunol."}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00769-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00769-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T03:54:23Z","timestamp":1684641263000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00769-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2]]},"references-count":51,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1128\/IAI.00769-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.00769-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2]]},"assertion":[{"value":"2008-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-10-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}