{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:04:51Z","timestamp":1790964291142,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Citrobacter rodentium<\/jats:italic>\n            is an attaching and effacing pathogen which causes transmissible colonic hyperplasia in mice. Infection with\n            <jats:italic>C. rodentium<\/jats:italic>\n            serves as a model for infection of humans with enteropathogenic and enterohemorrhagic\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . To identify novel colonization factors of\n            <jats:italic>C. rodentium<\/jats:italic>\n            , we screened a signature-tagged mutant library of\n            <jats:italic>C. rodentium<\/jats:italic>\n            in mice. One noncolonizing mutant had a single transposon insertion in an open reading frame (ORF) which we designated\n            <jats:italic>regA<\/jats:italic>\n            because of its homology to genes encoding members of the AraC family of transcriptional regulators. Deletion of\n            <jats:italic>regA<\/jats:italic>\n            in\n            <jats:italic>C. rodentium<\/jats:italic>\n            resulted in markedly reduced colonization of the mouse intestine. Examination of\n            <jats:italic>lacZ<\/jats:italic>\n            transcriptional fusions using promoter regions of known and putative virulence-associated genes of\n            <jats:italic>C. rodentium<\/jats:italic>\n            revealed that RegA strongly stimulated transcription of two newly identified genes located close to\n            <jats:italic>regA<\/jats:italic>\n            , which we designated\n            <jats:italic>adcA<\/jats:italic>\n            and\n            <jats:italic>kfcC<\/jats:italic>\n            . The cloned\n            <jats:italic>adcA<\/jats:italic>\n            gene conferred autoaggregation and adherence to mammalian cells to\n            <jats:italic>E. coli<\/jats:italic>\n            strain DH5\u03b1, and a\n            <jats:italic>kfc<\/jats:italic>\n            mutation led to a reduction in the duration of intestinal colonization, but the\n            <jats:italic>kfc<\/jats:italic>\n            mutant was far less attenuated than the\n            <jats:italic>regA<\/jats:italic>\n            mutant. These results indicated that other genes of\n            <jats:italic>C. rodentium<\/jats:italic>\n            whose expression required activation by RegA were required for colonization. Microarray analysis revealed a number of RegA-regulated ORFs encoding proteins homologous to known colonization factors. Transcription of these putative virulence determinants was activated by RegA only in the presence of sodium bicarbonate. Taken together, these results show that RegA is a global regulator of virulence in\n            <jats:italic>C. rodentium<\/jats:italic>\n            which activates factors that are required for intestinal colonization.\n          <\/jats:p>","DOI":"10.1128\/iai.00770-08","type":"journal-article","created":{"date-parts":[[2008,9,3]],"date-time":"2008-09-03T01:11:35Z","timestamp":1220404295000},"page":"5247-5256","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in\n            <i>Citrobacter rodentium<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Emily","family":"Hart","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia"},{"name":"Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia"},{"name":"Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Tauschek","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia"},{"name":"Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Wakefield","sequence":"additional","affiliation":[{"name":"Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gad","family":"Frankel","sequence":"additional","affiliation":[{"name":"Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth L.","family":"Hartland","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The University of Melbourne, Victoria 3010, Australia"},{"name":"Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy 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