{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:21:24Z","timestamp":1790695284409,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI099222"],"award-info":[{"award-number":["R01AI099222"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Murine Ly6C\n            <jats:sup>hi<\/jats:sup>\n            inflammatory monocytes (IMs) require CCR2 to leave the bone marrow and enter mesenteric lymph nodes (MLNs) and other organs in response to\n            <jats:named-content content-type=\"genus-species\">Yersinia pseudotuberculosis<\/jats:named-content>\n            infection. We are investigating how IMs, which can differentiate into CD11c\n            <jats:sup>+<\/jats:sup>\n            dendritic cells (DCs), contribute to innate and adaptive immunity to\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            . Previously, we obtained evidence that IMs are important for a dominant CD8\n            <jats:sup>+<\/jats:sup>\n            T cell response to the epitope YopE\n            <jats:sub>69\u201377<\/jats:sub>\n            and host survival using intravenous infections with attenuated\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            . Here we challenged CCR2\n            <jats:sup>+\/+<\/jats:sup>\n            or CCR2\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice orally with wild-type\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            to investigate how IMs contribute to immune responses during intestinal infection. Unexpectedly, CCR2\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice did not have reduced survival but retained body weight better and their MLNs cleared\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            faster and with reduced lymphadenopathy compared to controls. Enhanced bacterial clearance in CCR2\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice correlated with reduced numbers of IMs in spleens and increased numbers of neutrophils in livers.\n            <jats:italic>In situ<\/jats:italic>\n            imaging of MLNs and spleens from CCR2-GFP mice showed that green fluorescent protein-positive (GFP\n            <jats:sup>+<\/jats:sup>\n            ) IMs accumulated at the periphery of neutrophil-rich\n            <jats:named-content content-type=\"genus-species\">Yersinia-<\/jats:named-content>\n            containing pyogranulomas. GFP\n            <jats:sup>+<\/jats:sup>\n            IMs colocalized with CD11c\n            <jats:sup>+<\/jats:sup>\n            cells and YopE\n            <jats:sub>69\u201377<\/jats:sub>\n            -specific CD8\n            <jats:sup>+<\/jats:sup>\n            T cells in MLNs, suggesting that IM-derived DCs prime adaptive responses in\n            <jats:named-content content-type=\"genus-species\">Yersinia<\/jats:named-content>\n            pyogranulomas. Consistently, CCR2\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            mice had reduced numbers of splenic DCs, YopE\n            <jats:sub>69\u201377<\/jats:sub>\n            -specific CD8\n            <jats:sup>+<\/jats:sup>\n            T cells, CD4\n            <jats:sup>+<\/jats:sup>\n            T cells, and B cells in organs and lower levels of serum antibodies to\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            antigens. Our data suggest that IMs differentiate into DCs in MLN pyogranulomas and direct adaptive responses in T cells at the expense of innate immunity during oral\n            <jats:named-content content-type=\"genus-species\">Y. pseudotuberculosis<\/jats:named-content>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00782-17","type":"journal-article","created":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T13:34:03Z","timestamp":1513344843000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["CCR2\n            <sup>+<\/sup>\n            Inflammatory Monocytes Are Recruited to Yersinia pseudotuberculosis Pyogranulomas and Dictate Adaptive Responses at the Expense of Innate Immunity during Oral Infection"],"prefix":"10.1128","volume":"86","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Khairallah","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian S.","family":"Sheridan","sequence":"additional","affiliation":[{"name":"Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA"},{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrianus W. 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