{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T07:39:20Z","timestamp":1753601960868},"reference-count":37,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            , one of the causative agents of human brucellosis, causes acute, chronic, and relapsing infection. While T cell immunity in brucellosis has been extensively studied in mice, no recognized human T cell epitopes that might provide new approaches to classifying and prognosticating\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            infection have ever been delineated. Twenty-seven pools of 500 major histocompatibility complex class II (MHC-II) restricted peptides were created by computational prediction of promiscuous MHC-II CD4\n            <jats:sup>+<\/jats:sup>\n            T cell derived from the top 50 proteins recognized by IgG in human sera on a genome level\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            protein microarray. Gamma interferon (IFN-\u03b3) and interleukin-5 (IL-5) enzyme-linked immunospot (ELISPOT) analyses were used to quantify and compare Th1 and Th2 responses of leukapheresis-obtained peripheral blood mononuclear cells from Peruvian subjects cured after acute infection (\n            <jats:italic>n<\/jats:italic>\n            = 9) and from patients who relapsed (\n            <jats:italic>n<\/jats:italic>\n            = 5). Four peptide epitopes derived from 3\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            proteins (BMEI 1330, a DegP\/HtrA protease; BMEII 0029, type IV secretion system component VirB5; and BMEII 0691, a predicted periplasmic binding protein of a peptide transport system) were found repeatedly to produce significant IFN-\u03b3 ELISPOT responses in both acute-infection and relapsing patients; none of the peptides distinguished the patient groups. IL-5 responses against the panel of peptides were insignificant. These experiments are the first to systematically identify\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            MHC-II-restricted CD4\n            <jats:sup>+<\/jats:sup>\n            T cell epitopes recognized by the human immune response, with the potential for new approaches to brucellosis diagnostics and understanding the immunopathogenesis related to this intracellular pathogen.\n          <\/jats:p>","DOI":"10.1128\/iai.00796-13","type":"journal-article","created":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T00:16:11Z","timestamp":1381796171000},"page":"124-131","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Brucella melitensis T Cell Epitope Recognition in Humans with Brucellosis in Peru"],"prefix":"10.1128","volume":"82","author":[{"given":"Anthony P.","family":"Cannella","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014San Diego, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia S. Lindestam","family":"Arlehamn","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Sidney","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailash P.","family":"Patra","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014San Diego, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Torres","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Philosophy, Laboratorio de Investigacion y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renee M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of California\u2014Davis, Davis, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip L.","family":"Felgner","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayuko","family":"Saito","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014San Diego, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Gotuzzo","sequence":"additional","affiliation":[{"name":"Alexander von Humboldt Institute of Tropical Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Hospital Nacional Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"La Jolla Institute of Allergy and Immunology, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M.","family":"Vinetz","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California\u2014San Diego, La Jolla, California, USA"},{"name":"Faculty of Sciences and Philosophy, Laboratorio de Investigacion y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Alexander von Humboldt Institute of Tropical Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05298-11"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001929"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/588482"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02159-08"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7297-7303.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00994-09"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034925"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406873102"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00982.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2005.11.024"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.02.016"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00637-07"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.10.015"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2005.04.001"},{"key":"e_1_3_3_17_2","volume-title":"Brucella molecular and cellular microbiology, vol 1","author":"Lopez-Go\u00f1i I","year":"2004","unstructured":"Lopez-Go\u00f1iIMI. 2004. Brucella molecular and cellular microbiology, vol 1. Horizon Bioscience, Wymondham, England."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000673"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr200619r"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-9-801"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.02.008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2012.00001"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1103556"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn254"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000405"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.04.018"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003130"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00761-12"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.2019"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-409-8_15"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200850"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.32.1.2190"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00325-10"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-1852"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00406-08"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.04.036"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034246"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.01.001"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00796-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00796-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:33:10Z","timestamp":1646483590000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00796-13"}},"subtitle":[],"editor":[{"given":"R. P.","family":"Morrison","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1128\/IAI.00796-13"],"URL":"https:\/\/doi.org\/10.1128\/iai.00796-13","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1]]},"assertion":[{"value":"2013-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}