{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:49Z","timestamp":1787016649337,"version":"build-2736575974"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Melioidosis is a severe infectious disease caused by the saprophytic facultative intracellular pathogen\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    . The disease is endemic in Southeast Asia and Northern Australia, and no effective vaccine exists. To describe human cell-mediated immune responses to\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    and to identify candidate antigens for vaccine development, the ability of antigen-pulsed monocyte-derived dendritic cells (moDCs) to trigger autologous T-cell responses to\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    and its products was tested. moDCs were prepared from healthy individuals exposed or not exposed to\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    , based on serological evidence. These were pulsed with heat-killed\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    or purified antigens, including ABC transporters (LolC, OppA, and PotF), Bsa type III secreted proteins (BipD and BopE), tandem repeat sequence-containing proteins (Rp1 and Rp2), flagellin, and heat shock proteins (Hsp60 and Hsp70), prior to being mixed with autologous T-cell populations. After pulsing of cells with either heat-killed\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    , LolC, or Rp2, coculturing the antigen-pulsed moDCs with T cells elicited gamma interferon production from CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells from seropositive donors at levels greater than those for seronegative donors. These antigens also induced granzyme B (cytotoxic) responses from CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Activation of antigen-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells required direct contact with moDCs and was therefore not dependent on soluble mediators. Rp peptide epitopes recognized by T cells in healthy individuals were identified. Our study provides valuable novel data on the induction of human cell-mediated immune responses to\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    and its protein antigens that may be exploited in the rational development of vaccines to combat melioidosis.\n                  <\/jats:p>","DOI":"10.1128\/iai.00803-10","type":"journal-article","created":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T23:17:34Z","timestamp":1288653454000},"page":"305-313","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["<i>Burkholderia pseudomallei<\/i>\n                    Proteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T Cells\n                    <i>In Vitro<\/i>"],"prefix":"10.1128","volume":"79","author":[{"given":"Patcharaporn","family":"Tippayawat","sequence":"first","affiliation":[{"name":"The Centre for Research &amp; Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maneerat","family":"Pinsiri","sequence":"additional","affiliation":[{"name":"The Centre for Research &amp; Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darawan","family":"Rinchai","sequence":"additional","affiliation":[{"name":"The Centre for Research &amp; Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donporn","family":"Riyapa","sequence":"additional","affiliation":[{"name":"The Centre for Research &amp; Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amornrat","family":"Romphruk","sequence":"additional","affiliation":[{"name":"Blood Transfusion Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunn-Hwen","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond L.","family":"Houghton","sequence":"additional","affiliation":[{"name":"InBios International Inc., Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip L.","family":"Felgner","sequence":"additional","affiliation":[{"name":"University of California, Irvine, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Institute of Animal Health, Compton Laboratory, Compton, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard E.","family":"Galyov","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Bancroft","sequence":"additional","affiliation":[{"name":"Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganjana","family":"Lertmemongkolchai","sequence":"additional","affiliation":[{"name":"The Centre for Research &amp; Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2004.06.006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01447.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-10-20"},{"key":"e_1_3_2_5_2","first-page":"698","volume":"23","year":"1992","unstructured":"Charoenwong, P., P. 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