{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:21:09Z","timestamp":1791566469475,"version":"4.3.4"},"reference-count":82,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sal4 is a monoclonal polymeric IgA antibody directed against the O antigen (O-Ag) of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Salmonella enterica<\/jats:named-content>\n            serovar Typhimurium (\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium), which is sufficient to protect mice against intestinal infections from\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium. We recently reported that the exposure of\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium to Sal4 results in the immediate loss of flagellum-based motility, in alterations to the outer membrane (OM) integrity, and in the concomitant appearance of a mucoid phenotype that is reminiscent of cells in the earliest stages of biofilm formation. We demonstrate here that prolonged (&gt;4 h) exposure of\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium to Sal4 at 37\u00b0C (but not at ambient temperature [25\u00b0C]) results in measurable exopolysaccharide (EPS) accumulation and biofilm formation on both borosilicate glass surfaces and polystyrene microtiter plates. The polysaccharide produced by\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium in response to Sal4 contains cellulose, in addition to O-Ag capsule and colanic acid. EPS production was dependent on YeaJ, a proposed inner membrane-localized diguanylate cyclase (DGC) and a known regulator of cellulose biosynthesis. An\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium \u0394\n            <jats:italic>yeaJ<\/jats:italic>\n            strain was unable to produce cellulose or form a biofilm in response to Sal4. Conversely, the overexpression of\n            <jats:italic>yeaJ<\/jats:italic>\n            in\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium enhanced Sal4-induced biofilm formation and resulted in increased intracellular levels of cyclic dimeric guanosine monophosphate (c-di-GMP) compared to that of a wild-type control; this strongly suggests that YeaJ is indeed a functional DGC. Based on these data, we speculate that Sal4, by virtue of its ability to associate with the O-Ag and to induce OM stress, renders\n            <jats:italic>S<\/jats:italic>\n            . Typhimurium avirulent by triggering a c-di-GMP-dependent signaling pathway via YeaJ that leads to the suppression of bacterial motility while simultaneously stimulating EPS production.\n          <\/jats:p>","DOI":"10.1128\/iai.00813-12","type":"journal-article","created":{"date-parts":[[2012,12,11]],"date-time":"2012-12-11T04:43:06Z","timestamp":1355200986000},"page":"653-664","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Exposure of Salmonella enterica Serovar Typhimurium to a Protective Monoclonal IgA Triggers Exopolysaccharide Production via a Diguanylate Cyclase-Dependent Pathway"],"prefix":"10.1128","volume":"81","author":[{"given":"Jayaleka J.","family":"Amarasinghe","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca E.","family":"D'Hondt","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Waters","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Mantis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, New York, USA"},{"name":"Biomedical Sciences Program, University at Albany School of Public Health, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01375-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006098107"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4879-4885.1999"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3843-3855.2002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3219-3227.2005"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.16.6383"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06056.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.6.2593"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.12.002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.05.001"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01312.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00786-08"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00791.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.d01-1718.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81003-X"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4654-4661.2004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2109"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.40.110405.090423"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.12.008"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2011.01.038"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028351"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.41"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.5.1786-1792.1992"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(94)90214-3"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1615-1618.2002"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00416-08"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/08820131003622635"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00018-12"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02337.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2640-2649.2002"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00809-06"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-3074-5"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02088-05"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044841"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2012.00645.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)36577-1"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00798-06"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2001.00213.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02802.x"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.3.722-731.1998"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.24.8213-8220.2004"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00126-09"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01756-07"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07470.x"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04737.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.5904-5909.1996"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.6.1411-1412.1989"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/35101614"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-4557-x"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04269.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90366-J"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00493.x"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01822.x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.12.5279-5285.1993"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052093"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2009.03.013"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01569816"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.7154-7158.2003"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03070.x"},{"key":"e_1_3_3_62_2","first-page":"127","article-title":"Biofilms of Salmonella enterica","author":"R\u00f6mling U","year":"2007","unstructured":"R\u00f6mlingUYaronPDS. 2007. Biofilms of Salmonella enterica, p 127\u2013145. In RhenDMMMastroeniPThrelfallEJ (ed), Salmonella molecular biology and pathogenesis. Horizon Press, Norfolk, United Kingdom.","journal-title":"Salmonella molecular biology and pathogenesis"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2004.01513.x"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.3214-3226.2005"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01612.x"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04907.x"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01428-05"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05123.x"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2001.00235.x"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.944-949.1998"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00268"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2003.01700.x"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.21.5946-5954.2002"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02032.x"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01719-06"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04206.x"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812573106"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-3-1"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01672-8"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01887-05"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10814.x"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2011.03411.x"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901838"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00813-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00813-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:23:16Z","timestamp":1646482996000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00813-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":82,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1128\/IAI.00813-12"],"URL":"https:\/\/doi.org\/10.1128\/iai.00813-12","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]},"assertion":[{"value":"2012-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}