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In this study, we infected BALB\/c mice with infective stage 3 larvae of the lymphatic filarial parasite\n            <jats:named-content content-type=\"genus-species\">Brugia malayi<\/jats:named-content>\n            (Bm-L3) and studied the effect on fluorescence-activated cell sorter (FACS)-sorted DC subsets. While myeloid DCs (mDCs) accumulated by day 3 postinfection (p.i.), lymphoid DCs (LDCs) and CD8\n            <jats:sup>+<\/jats:sup>\n            plasmacytoid DCs (pDCs) peaked at day 7 p.i. in the spleens and mesenteric lymph nodes (mLNs) of infected mice. Increased tumor necrosis factor alpha (TNF-\u03b1) but reduced interleukin 12 (IL-12) and Toll-like receptor 4 (TLR4), -6, and -9 and reciprocal secretion of IL-4 and IL-10 were also observed across all DC subsets. Interestingly, Bm-L3 increased the expression of CD80 and CD86 across all DC subsets but decreased that of major histocompatibility complex class II (MHC-II) on mDCs and pDCs, resulting in their impaired antigen uptake and presentation capacities, but maximally attenuated the T-cell proliferation capacity of only mDCs. Furthermore, Bm-L3 increased phosphorylated p38 (p-p38), but not p-ERK, in mDCs and LDCs but downregulated them in pDCs, along with differential modulation of protein tyrosine phosphatases SHP-1, TCPTP, PTEN, and PTP1B across all DC subsets. Taken together, we report hitherto undocumented effects of early Bm-L3 infection on purified host DC subsets that lead to their functional impairment and attenuated host T-cell response.\n          <\/jats:p>","DOI":"10.1128\/iai.00818-16","type":"journal-article","created":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T01:12:21Z","timestamp":1477962741000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Functional Impairment of Murine Dendritic Cell Subsets following Infection with Infective Larval Stage 3 of Brugia malayi"],"prefix":"10.1128","volume":"85","author":[{"given":"Aditi","family":"Sharma","sequence":"first","affiliation":[{"name":"Parasitology Division, CSIR-Central Drug Research Institute, Lucknow, India"},{"name":"Academy of Scientific and Innovative Research (AcSIR), New Delhi, 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