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We initially identified BB0744 (also known as p83\/100) by screening for<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>strain B31 proteins that bind to \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>integrin and hypothesized that, given the presence of a signal peptide, BB0744 may be a surface-exposed protein. In contrast to this expectation, localization studies suggested that BB0744 resides in the periplasm. Despite its subsurface location, we were interested in testing whether BB0744 is required for borrelial pathogenesis. To this end, a<jats:italic>bb0744<\/jats:italic>deletion was isolated in a<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>strain B31 infectious background, complemented, and queried for the role of BB0744 following experimental infection. A combination of bioluminescent imaging, cultivation of infected tissues, and quantitative PCR (qPCR) demonstrated that \u0394<jats:italic>bb0744<\/jats:italic>mutant<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>bacteria were attenuated in the ability to colonize heart tissue, as well as skin locations distal to the site of infection. Furthermore, qPCR indicated a significantly reduced spirochetal load in distal skin and joint tissue infected with \u0394<jats:italic>bb0744<\/jats:italic>mutant<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>. Complementation with<jats:italic>bb0744<\/jats:italic>restored infectivity, indicating that the defect seen in \u0394<jats:italic>bb0744<\/jats:italic>mutant<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>was due to the loss of BB0744. Taken together, these results suggest that BB0744 is necessary for tissue tropism, particularly in heart tissue, alters the ability of<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>to disseminate efficiently, or both. Additional studies are warranted to address the mechanism employed by BB0744 that alters the pathogenic potential of<jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/iai.00828-15","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T02:30:36Z","timestamp":1436236236000},"page":"3693-3703","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["BB0744 Affects Tissue Tropism and Spatial Distribution of Borrelia burgdorferi"],"prefix":"10.1128","volume":"83","author":[{"given":"Beau","family":"Wager","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&amp;M Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana K.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&amp;M Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley M.","family":"Groshong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon S.","family":"Blevins","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon T.","family":"Skare","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&amp;M Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.278331"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu397"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1314325"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2714"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21681"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60103-7"},{"key":"e_1_3_3_8_2","first-page":"993","article-title":"Three sudden cardiac deaths associated with Lyme carditis\u2014United States, November 2012\u2013July 2013","volume":"62","author":"Centers for Disease Control and Prevention (CDC)","year":"2013","unstructured":"Centers for Disease Control and Prevention (CDC). 2013. 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