{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:58:33Z","timestamp":1790812713621,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI139242"],"award-info":[{"award-number":["AI139242"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI065530"],"award-info":[{"award-number":["AI065530"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI124649"],"award-info":[{"award-number":["AI124649"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2020,4,20]]},"abstract":"<jats:p>\n            The translocated actin recruiting phosphoprotein (Tarp) is a multidomain type III secreted effector used by\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            . In aggregate, existing data suggest a role of this effector in initiating new infections. As new genetic tools began to emerge to study chlamydial genes\n            <jats:italic>in vivo<\/jats:italic>\n            , we speculated as to what degree Tarp function contributes to\n            <jats:italic>Chlamydia<\/jats:italic>\n            \u2019s ability to parasitize mammalian host cells. To address this question, we generated a complete\n            <jats:italic>tarP<\/jats:italic>\n            deletion mutant using the fluorescence-reported allelic exchange mutagenesis (FRAEM) technique and complemented the mutant in\n            <jats:italic>trans<\/jats:italic>\n            with wild-type\n            <jats:italic>tarP<\/jats:italic>\n            or mutant\n            <jats:italic>tarP<\/jats:italic>\n            alleles engineered to harbor in-frame domain deletions.\n          <\/jats:p>","DOI":"10.1128\/iai.00841-19","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T10:39:06Z","timestamp":1583404746000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Fluorescence-Reported Allelic Exchange Mutagenesis-Mediated Gene Deletion Indicates a Requirement for Chlamydia trachomatis Tarp during\n            <i>In Vivo<\/i>\n            Infectivity and Reveals a Specific Role for the C Terminus during Cellular Invasion"],"prefix":"10.1128","volume":"88","author":[{"given":"Susmita","family":"Ghosh","sequence":"first","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Ruelke","sequence":"additional","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua C.","family":"Ferrell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology &amp; Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria D.","family":"Bodero","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology &amp; Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth A.","family":"Fields","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology &amp; Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis J.","family":"Jewett","sequence":"additional","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"729","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"Moulder JW","year":"1984","unstructured":"Moulder JW, Hatch TP, Kuo CC, Schachter J, Storz J. 1984. 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