{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:52Z","timestamp":1790665552922,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2009,3,1]],"date-time":"2009-03-01T00:00:00Z","timestamp":1235865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Mycoplasma genitalium<\/jats:italic>has been implicated in several important reproductive tract syndromes in women, including pelvic inflammatory disease, cervicitis, and tubal factor infertility. The mechanisms of immune activation are unclear, and we sought to determine whether<jats:italic>M. genitalium<\/jats:italic>was capable of activating innate immune responses through ligation of highly expressed Toll-like receptors (TLR) of the genital tract. Using HEK293 cells expressing specific human TLR, viable<jats:italic>M. genitalium<\/jats:italic>and the recombinant C-terminal portion of the immunogenic protein MG309 (rMG309c) were shown to activate NF-\u03baB via TLR2\/6. These data provided a putative mechanism for activation of the innate response in genital tissues. Genital epithelial cells (EC) are the first responders to sexually transmitted pathogens and express high levels of TLR2 and -6. Following exposure to purified rMG309c, vaginal and ecto- and endocervical EC secreted proinflammatory cytokines, including interleukin-6 (IL-6) and IL-8. Vaginal EC were less responsive than cervical EC. The capacity of rMG309c to bind TLR2\/6 and elicit inflammation was sensitive to proteinase K digestion and independent of traditional N-terminal lipoylation. Furthermore, the immunostimulatory capacity of rMG309c was localized specifically to a 91-amino-acid subfragment of the recombinant protein, suggesting that TLR activation is likely amino acid based. Together, these data indicated that human vaginal and cervical EC are immunologically responsive to<jats:italic>M. genitalium<\/jats:italic>and to purified rMG309c via highly expressed TLR of the genital tract. These findings provide valuable insights into the mechanisms for activation of acute-phase inflammatory responses and suggest that<jats:italic>M. genitalium<\/jats:italic>colonization of reproductive tract tissues may result in inflammatory sequelae.<\/jats:p>","DOI":"10.1128\/iai.00845-08","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T01:45:28Z","timestamp":1229996728000},"page":"1175-1181","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["<i>Mycoplasma genitalium<\/i>-Encoded MG309 Activates NF-\u03baB via Toll-Like Receptors 2 and 6 To Elicit Proinflammatory Cytokine Secretion from Human Genital Epithelial Cells"],"prefix":"10.1128","volume":"77","author":[{"given":"Chris L.","family":"McGowin","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-0609"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Martin","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B.","family":"Pyles","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-0609"},{"name":"Department of Pediatrics, Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas 77555-0436"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80909-Z"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/154.4_Pt_2.S137"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69848-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512525200"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05029.x"},{"key":"e_1_3_2_7_2","first-page":"31","volume":"4","year":"2007","unstructured":"Carmody, R. 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