{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:28:22Z","timestamp":1791073702151,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pneumonic plague, caused by inhalation of\n            <jats:italic>Yersinia pestis<\/jats:italic>\n            , represents a major bioterrorism threat for which no vaccine is available. Based on the knowledge that genetic delivery of monoclonal antibodies (MAbs) with adenovirus (Ad) gene transfer vectors results in rapid, high-level antibody expression, we evaluated the hypothesis that Ad-mediated delivery of a neutralizing antibody directed against the\n            <jats:italic>Y. pestis<\/jats:italic>\n            V antigen would protect mice against a\n            <jats:italic>Y. pestis<\/jats:italic>\n            challenge. MAbs specific for the\n            <jats:italic>Y. pestis<\/jats:italic>\n            V antigen were generated, and the most effective in protecting mice against a lethal intranasal\n            <jats:italic>Y. pestis<\/jats:italic>\n            challenge was chosen for further study. The coding sequences for the heavy and light chains were isolated from the corresponding hybridoma and inserted into a replication-defective serotype 5 human Ad gene transfer vector (Ad\u03b1V). Western analysis of Ad\u03b1V-infected cell supernatants demonstrated completely assembled antibodies reactive with V antigen. Following Ad\u03b1V administration to mice, high levels of anti-V antigen antibody titers were detectable as early as 1 day postadministration, peaked by day 3, and remained detectable through a 12-week time course. When animals that received Ad\u03b1V were challenged with\n            <jats:italic>Y. pestis<\/jats:italic>\n            at day 4 post-Ad\u03b1V administration, 80% of the animals were protected, while 0% of control animals survived (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). Ad-mediated delivery of a V antigen-neutralizing antibody is an effective therapy against plague in experimental animals and could be developed as a rapidly acting antiplague therapeutic.\n          <\/jats:p>","DOI":"10.1128\/iai.00856-08","type":"journal-article","created":{"date-parts":[[2009,1,6]],"date-time":"2009-01-06T01:34:03Z","timestamp":1231205643000},"page":"1561-1568","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Adenovirus-Mediated Delivery of an Anti-V Antigen Monoclonal Antibody Protects Mice against a Lethal\n            <i>Yersinia pestis<\/i>\n            Challenge"],"prefix":"10.1128","volume":"77","author":[{"given":"Carolina","family":"Sofer-Podesta","sequence":"first","affiliation":[{"name":"Department of Genetic Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ang","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil R.","family":"Hackett","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Senina","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Perlin","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Newark, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald G.","family":"Crystal","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie L.","family":"Boyer","sequence":"additional","affiliation":[{"name":"Department of Genetic Medicine, Weill Cornell Medical College, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.793"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.11.4580-4585.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.56.471"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2180-2187.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01497-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00317-5"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro974"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/507644"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300344"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1087"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300142"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(86)90142-8"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(87)51008-4"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199709043371004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.09.020"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1999.tb01229.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00299-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)80110-2"},{"key":"e_1_3_2_20_2","first-page":"124","volume":"2","year":"1995","unstructured":"Hersh, J., R. G. Crystal, and B. Bewig. 1995. Modulation of gene expression after replication-deficient, recombinant adenovirus-mediated gene transfer by the product of a second adenovirus vector. Gene Ther.2:124-131.","journal-title":"Gene Ther."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.2234-2238.2003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.3068-3070.2006"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.11.4476-4482.1997"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.17.2281"},{"key":"e_1_3_2_25_2","first-page":"CD000976","volume":"2","year":"2000","unstructured":"Jefferson, T., V. Demicheli, and M. Pratt. 2000. Vaccines for preventing plague. Cochrane Database Syst. Rev.2:CD000976.","journal-title":"Cochrane Database Syst. Rev."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0746"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.10.018"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.8.2854-2858.1995"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.17.8769-8775.2002"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8695"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00073-07"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.11.7498-7509.1996"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(01)00353-2"},{"key":"e_1_3_2_34_2","first-page":"4192","volume":"2","year":"1994","unstructured":"Motin, V. L., R. Nakajima, G. B. Smirnov, and R. R. Brubaker. 1994. Passive immunity to yersiniae mediated by anti-recombinant V antigen and protein A-V antigen fusion peptide. Infect. Immun.2:4192-4201.","journal-title":"Infect. Immun."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430340260185111"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.35"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01408.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1532-1542.2005"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01644-06"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60566-2"},{"key":"e_1_3_2_41_2","first-page":"37","volume":"56","year":"1989","unstructured":"Rasoamanana, B., P. Coulanges, P. Michel, and N. Rasolofonirina. 1989. Sensitivity of Yersinia pestis to antibiotics: 277 strains isolated in Madagascar between 1926 and 1989. Arch. Inst. Pasteur Madagascar56:37-53. (In French.)","journal-title":"Arch. Inst. Pasteur Madagascar"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.04.003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90213-V"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00090-N"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.5.1207-1214.1998"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","unstructured":"Skaricic D. C. Traube B. De J. Joh J. Boyer R. G. Crystal and S. Worgall. 2008. Genetic delivery of an anti-RSV antibody to protect against pulmonary infection with RSV. Virology [Epub ahead of print.] doi:10.1016\/j.virol.2008.04.016.","DOI":"10.1016\/j.virol.2008.04.016"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050219"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0310104"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00163-3"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.4.6.965"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.09.021"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.02.036"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01497.x"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00232-3"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00196.x"},{"key":"e_1_3_2_56_2","first-page":"566","volume":"19","year":"2001","unstructured":"Williamson, E. D., S. M. Eley, A. J. Stagg, M. Green, P. Russell, and R. W. Titball. 2001. A single dose sub-unit vaccine protects against pneumonic plague. Vaccine19:566-571.","journal-title":"Vaccine"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00355-5"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.06.006"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00856-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00856-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:55:31Z","timestamp":1646466931000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00856-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1128\/IAI.00856-08"],"URL":"https:\/\/doi.org\/10.1128\/iai.00856-08","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4]]},"assertion":[{"value":"2008-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-12-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}