{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:26:27Z","timestamp":1791152787232,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In addition to the yersiniabactin (Ybt) siderophore-dependent system, two inorganic iron ABC transport systems of\n                    <jats:italic>Yersinia pestis<\/jats:italic>\n                    , Yfe and Yfu, have been characterized. Here we show that the Yfu system functions in\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    : a Ybt\n                    <jats:sup>\u2212<\/jats:sup>\n                    Yfe\n                    <jats:sup>\u2212<\/jats:sup>\n                    Yfu\n                    <jats:sup>\u2212<\/jats:sup>\n                    mutant exhibited a greater growth defect under iron-deficient conditions than its Ybt\n                    <jats:sup>\u2212<\/jats:sup>\n                    Yfe\n                    <jats:sup>\u2212<\/jats:sup>\n                    parental strain. We also demonstrate that another putative\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    iron uptake system, Yiu, which potentially encodes an outer membrane receptor, YiuR, and an ABC iron transport cassette, YiuABC, is functional. The cloned\n                    <jats:italic>yiuABC<\/jats:italic>\n                    operon restored growth of an enterobactin-deficient mutant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strain, 1017, under iron-chelated conditions. Iron uptake by the Yiu system in\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    was demonstrated only when the Ybt, Yfe, and Yfu systems were mutated. Using a\n                    <jats:italic>yiuA<\/jats:italic>\n                    ::\n                    <jats:italic>lacZ<\/jats:italic>\n                    fusion, we show that the\n                    <jats:italic>yiuABC<\/jats:italic>\n                    promoter is repressed by iron through Fur. A mouse model of bubonic plague failed to show a significant role for the Yiu system in the disease process. These results demonstrate that two additional iron transporters are functional in\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    and indicate that there is a hierarchy of iron transporters, with Ybt being most effective and Yiu being the least effective of those systems which have been characterized.\n                  <\/jats:p>","DOI":"10.1128\/iai.00874-06","type":"journal-article","created":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T18:57:47Z","timestamp":1161370667000},"page":"6171-6178","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Hierarchy of Iron Uptake Systems: Yfu and Yiu Are Functional in\n                    <i>Yersinia pestis<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"Olga","family":"Kirillina","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Bobrov","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline D.","family":"Fetherston","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel F. 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