{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:24:26Z","timestamp":1782257066744,"version":"3.54.5"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The autoinducer-2 (AI-2) quorum-sensing system has been linked to diverse phenotypes and regulatory changes in pathogenic bacteria. In the present study, we performed a molecular and biochemical characterization of the AI-2 system in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Yersinia pestis<\/jats:named-content>\n            , the causative agent of plague. In strain CO92, the AI-2 signal is produced in a\n            <jats:italic>luxS<\/jats:italic>\n            -dependent manner, reaching maximal levels of 2.5 \u03bcM in the late logarithmic growth phase, and both wild-type and pigmentation (\n            <jats:italic>pgm<\/jats:italic>\n            ) mutant strains made equivalent levels of AI-2. Strain CO92 possesses a chromosomal\n            <jats:italic>lsr<\/jats:italic>\n            locus encoding factors involved in the binding and import of AI-2, and confirming this assignment, an\n            <jats:italic>lsr<\/jats:italic>\n            deletion mutant increased extracellular pools of AI-2. To assess the functional role of AI-2 sensing in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pestis<\/jats:named-content>\n            , microarray studies were conducted by comparing \u0394\n            <jats:italic>pgm<\/jats:italic>\n            strain R88 to a \u0394\n            <jats:italic>pgm<\/jats:italic>\n            \u0394\n            <jats:italic>luxS<\/jats:italic>\n            mutant or a quorum-sensing-null \u0394\n            <jats:italic>pgm<\/jats:italic>\n            \u0394\n            <jats:italic>ypeIR<\/jats:italic>\n            \u0394\n            <jats:italic>yspIR<\/jats:italic>\n            \u0394\n            <jats:italic>luxS<\/jats:italic>\n            mutant at 37\u00b0C. Our data suggest that AI-2 quorum sensing is associated with metabolic activities and oxidative stress genes that may help\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pestis<\/jats:named-content>\n            survive at the host temperature. This was confirmed by observing that the\n            <jats:italic>luxS<\/jats:italic>\n            mutant was more sensitive to killing by hydrogen peroxide, suggesting a potential requirement for AI-2 in evasion of oxidative damage. We also show that a large number of membrane protein genes are controlled by LuxS, suggesting a role for quorum sensing in membrane modeling. Altogether, this study provides the first global analysis of AI-2 signaling in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Y. pestis<\/jats:named-content>\n            and identifies potential roles for the system in controlling genes important to disease.\n          <\/jats:p>","DOI":"10.1128\/iai.00880-13","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T01:58:53Z","timestamp":1376963933000},"page":"4053-4062","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Analysis of Autoinducer-2 Quorum Sensing in Yersinia pestis"],"prefix":"10.1128","volume":"81","author":[{"given":"Jing","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa L.","family":"Madsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Carruthers","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey S.","family":"Kavanaugh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff M.","family":"Boyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander R.","family":"Horswill","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. Chris","family":"Minion","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/016154-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.Supplement_4.S748"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-72124-8_15"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/415545a"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb600444h"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.109"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00409093"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:32:1:043"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02532.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.07.020"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062337"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01587.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.160-166.2006"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1251\/bpo122"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01446.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03781.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02669.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-4-36"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06477.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0106"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00398-06"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.1.238-248.2005"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00387-07"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.25853-0"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.2.587-598.2006"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2007.0657"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1146"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1107\/S1744309111042953"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(03)00028-6"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.1.274-284.2003"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.35"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00727-09"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00880-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00880-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:25:06Z","timestamp":1646483106000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00880-13"}},"subtitle":[],"editor":[{"given":"A. J.","family":"B\u00e4umler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1128\/IAI.00880-13"],"URL":"https:\/\/doi.org\/10.1128\/iai.00880-13","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"2013-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}