{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:52:36Z","timestamp":1791165156609,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A biphasic developmental cycle whereby highly resistant small-cell variants (SCVs) are generated from large-cell variants (LCVs) is considered fundamental to the virulence of\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    , the causative agent of human Q fever. In this study a proteome analysis of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    developmental forms was conducted to provide insight into their unique biological and immunological properties. Silver-stained gels of SCV and LCV lysates separated by two-dimensional (2-D) gel electrophoresis resolved over 675 proteins in both developmental forms. Forty-eight proteins were greater than twofold more abundant in LCVs than in SCVs, with six proteins greater than twofold more abundant in SCVs than in LCVs. Four and 15 upregulated proteins of SCVs and LCVs, respectively, were identified by mass spectrometry, and their predicted functional roles are consistent with a metabolically active LCV and a structurally resistant SCV. One-dimensional and 2-D immunoblots of cell form lysates probed with sera from infected\/vaccinated guinea pigs and convalescent-phase serum from human patients who had recovered from acute Q fever, respectively, revealed both unique SCV\/LCV antigens and common SCV\/LCV antigens that were often differentially synthesized. Antigens recognized during human infection were identified by mass spectroscopy and included both previously described immunodominant proteins of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    and novel immunogenic proteins that may be important in the pathophysiology of clinical Q fever and\/or the induction of protective immunity.\n                  <\/jats:p>","DOI":"10.1128\/iai.00883-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T18:51:25Z","timestamp":1166640685000},"page":"290-298","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Proteome and Antigen Profiling of\n                    <i>Coxiella burnetii<\/i>\n                    Developmental Forms"],"prefix":"10.1128","volume":"75","author":[{"given":"Sherry A.","family":"Coleman","sequence":"first","affiliation":[{"name":"Coxiella Pathogenesis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth R.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Microscopy Unit, Research Technology Section, Research Technology Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane C.","family":"Cockrell","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Voth","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale","family":"Howe","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Mead","sequence":"additional","affiliation":[{"name":"Host-Parasite Interactions Section, Laboratory of Intracellular Parasites"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Samuel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Texas A&amp;M University System Health Science Center, College Station, Texas 77843-1114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Heinzen","sequence":"additional","affiliation":[{"name":"Coxiella Pathogenesis Section"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/138920305774933240"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2309-2324.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(03)00037-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-006-0097-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7344-7352.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/10409230490892496"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2004.03.007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28804-0"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.2.359-365.1985"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04567.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2006.03.002"},{"key":"e_1_3_2_13_2","first-page":"99","year":"1997","unstructured":"Heinzen, R. A. 1997. Intracellular development of Coxiella burnetii, p. 99-129. In B. Anderson, M. Bendinelli, and H. Friedman (ed.), Rickettsial infection and immunity. 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Ultrastructural and biological aspects of Coxiella burnetii under physical disruptions, p. 267-280. In W. Burgdorfer and R. L. Anacker (ed.), Rickettsiae and rickettsial diseases. Academic Press, New York, NY.","journal-title":"Rickettsiae and rickettsial diseases"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.147.3.1063-1076.1981"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.5.1144-1150.1987"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00334.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3304-3312.2004"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6026-6033.1999"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931379100"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4874-4883.2001"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2004.tb00580.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2005.08.004"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.13.4362-4371.2005"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.5.1454-1461.1992"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6741-6750.2002"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.19.12265"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.110.1.368-377.1972"},{"key":"e_1_3_2_37_2","first-page":"21","year":"1991","unstructured":"Williams, J. C. 1991. Infectivity, virulence, and pathogenicity of Coxiella burnetii for various hosts, p. 21-71. In J. C. Williams and H. A. Thompson (ed.), Q fever: the biology of Coxiella burnetii. 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