{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T15:30:26Z","timestamp":1771515026554,"version":"3.50.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            ToxR facilitates TcpP-mediated activation of the\n            <jats:italic>toxT<\/jats:italic>\n            promoter in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Vibrio cholerae<\/jats:named-content>\n            , initiating a regulatory cascade that culminates in cholera toxin secretion and toxin coregulated pilus expression. ToxR binds a region from \u2212104 to \u221268 of the\n            <jats:italic>toxT<\/jats:italic>\n            promoter, from which ToxR recruits TcpP to the TcpP-binding site from \u221253 to \u221238. To precisely define the ToxR-binding site within the\n            <jats:italic>toxT<\/jats:italic>\n            promoter, promoter derivatives with single-base-pair transversions spanning the ToxR-footprinted region were tested for transcription activation and DNA binding. Nine transversions between \u221296 to \u221283 reduced\n            <jats:italic>toxT<\/jats:italic>\n            promoter activity 3-fold or greater, and all nine reduced the relative affinity of the\n            <jats:italic>toxT<\/jats:italic>\n            promoter for ToxR at least 2-fold, indicating that activation defects were due largely to reduced binding of ToxR to the\n            <jats:italic>toxT<\/jats:italic>\n            promoter. Nucleotides important for ToxR-dependent\n            <jats:italic>toxT<\/jats:italic>\n            activation revealed a consensus sequence of TNAAA-N\n            <jats:sub>5<\/jats:sub>\n            -TNAAA extending from \u221296 to \u221283, also present in other ToxR-regulated promoters. When these consensus nucleotides were mutated in the\n            <jats:italic>ompU<\/jats:italic>\n            ,\n            <jats:italic>ompT<\/jats:italic>\n            , or\n            <jats:italic>ctxA<\/jats:italic>\n            promoters, ToxR-mediated regulation was disrupted. Thus, we have defined the core ToxR-binding site present in numerous ToxR-dependent promoters and we have precisely mapped the binding site for ToxR to a position three helical turns upstream of TcpP in the\n            <jats:italic>toxT<\/jats:italic>\n            promoter.\n          <\/jats:p>","DOI":"10.1128\/iai.00889-12","type":"journal-article","created":{"date-parts":[[2013,1,8]],"date-time":"2013-01-08T18:25:09Z","timestamp":1357669509000},"page":"884-895","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["ToxR Recognizes a Direct Repeat Element in the\n            <i>toxT<\/i>\n            ,\n            <i>ompU<\/i>\n            ,\n            <i>ompT<\/i>\n            , and\n            <i>ctxA<\/i>\n            Promoters of Vibrio cholerae To Regulate Transcription"],"prefix":"10.1128","volume":"81","author":[{"given":"Thomas 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