{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:27:09Z","timestamp":1792794429404,"version":"4.5.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Evasion of host immune defenses is critical for the progression of invasive infections caused by the leading neonatal pathogen, group B streptococcus (GBS). Upon characterizing the factors required for virulence in a neonatal rat sepsis model, we found that a surface-associated penicillin-binding protein (PBP1a), encoded by<jats:italic>ponA<\/jats:italic>, played an essential role in resistance of GBS to phagocytic clearance. In order to elucidate how PBP1a promotes resistance to innate immunity, we compared the susceptibility of wild-type GBS and an isogenic<jats:italic>ponA<\/jats:italic>mutant to the bactericidal components of human neutrophils. The isogenic strains were found to be equally capable of blocking complement activation on the bacterial surface and equally associated with phagocytes and susceptible to oxidative killing. In contrast, the<jats:italic>ponA<\/jats:italic>mutant was significantly more susceptible to killing by cationic antimicrobial peptides (AMPs) of the cathelicidin and defensin families, which are now recognized as integral components of innate host defense against invasive bacterial infection. These observations may help explain the sensitivity to phagocytic killing and attenuated virulence of the<jats:italic>ponA<\/jats:italic>mutant. This novel function for PBP1a in promoting resistance of GBS to AMP did not involve an alteration in bacterial surface charge or peptidoglycan cross-linking. While the peptidoglycan polymerization and cross-linking activity of PBPs are essential for bacterial survival, our study is the first to identify a role for a PBP in resistance to host AMPs.<\/jats:p>","DOI":"10.1128\/iai.00895-06","type":"journal-article","created":{"date-parts":[[2006,10,20]],"date-time":"2006-10-20T22:57:47Z","timestamp":1161385067000},"page":"6179-6187","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Penicillin-Binding Protein 1a Promotes Resistance of Group B Streptococcus to Antimicrobial Peptides"],"prefix":"10.1128","volume":"74","author":[{"given":"Andrea","family":"Hamilton","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Popham","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Carl","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Lauth","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Diego, La Jolla, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Nizet","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Diego, La Jolla, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198306000-00015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2054-2065.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.68.4.544"},{"key":"e_1_3_2_5_2","first-page":"1091","year":"2005","unstructured":"Baker, C. J., and M. W. Edwards. 2005. Group B streptococcal infections, p. 1091-1156. In J. S. Remington, J. O. Klein, C. B. Wilson, and C. J. Baker (ed.), Infectious diseases of the fetus and newborn infant, 6 ed. Elsevier\/Saunders, Philadelphia, Pa.","journal-title":"Infectious diseases of the fetus and newborn infant"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197604012941404"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.4049\/jimmunol.118.2.673","volume":"118","year":"1977","unstructured":"Baltimore, R. S., D. L. Kasper, C. J. Baker, and D. K. Goroff. 1977. Antigenic specificity of opsonophagocytic antibodies in rabbit anti-sera to group B streptococci. J. Immunol.118:673-678.","journal-title":"J. Immunol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.4049\/jimmunol.163.5.2761","volume":"163","year":"1999","unstructured":"Butko, P., A. Nicholson-Weller, and M. R. Wessels. 1999. Role of complement component C1q in the IgG-independent opsonophagocytosis of group B streptococcus. J. Immunol.163:2761-2768.","journal-title":"J. Immunol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.6.1978-1983.1991"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.2.572-581.1982"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.13.3981-3993.1999"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000057205.64451.B7"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4412-4416.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2394-2403.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.5.1275"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.9.3556-3565.1992"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5189"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1180"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.4.702-738.2002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1079-1093.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.4.697"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11554"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.11939"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.7.1831-1840.1998"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199957040-00002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108593"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6552-6555.1999"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09575.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02099.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03297.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.19.6719-6725.2005"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.142.1.165"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02335.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406143101"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00741.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.10.3986-3993.1992"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/165.2.306"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.20.6046-6053.2001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.16.4491-4499.2000"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5911966.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.4049\/jimmunol.118.6.2192","volume":"118","year":"1977","unstructured":"Minta, J. O., D. Man, and H. Z. Movat. 1977. Kinetic studies on the fragmentation of the third component of complement (C3) by trypsin. J. Immunol.118:2192-2198.","journal-title":"J. Immunol."},{"key":"e_1_3_2_43_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Performance standard for antimicrobial susceptibility testing: tenth informational supplement (aerobic dilution). Approved standard M100-S10. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540310015629"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/35106587"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02333-8"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.9.1067"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8405"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02428.x"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5620-5629.1998"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.2.326-335.1995"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.7.2079-2085.1996"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.21.6324-6334.2001"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5098-5106.2001"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03655.x"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-10-3245"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.20.7208"},{"key":"e_1_3_2_58_2","first-page":"22","volume":"14","year":"1990","unstructured":"Rubens, C. E., M. R. Wessels, J. M. Kuypers, D. L. Kasper, and J. N. Weiser. 1990. Molecular analysis of two group B streptococcal virulence factors. Semin. Perinatol.14:22-29.","journal-title":"Semin. Perinatol."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6026-6038.2005"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1086\/322697"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.21.1.34-40.1978"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.156.3772.213"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000180300045"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.4.1089-1094.1989"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8983"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.2.323"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.6.2.156"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12132.x"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11239-6"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.5319-5321.2002"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00895-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00895-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T13:18:18Z","timestamp":1683638298000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00895-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":69,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1128\/IAI.00895-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.00895-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]},"assertion":[{"value":"2006-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}