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We identified and altered the expression of the gene encoding a Rho-GDI homolog in the human fungal pathogen\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    and investigated its impact on pathogenicity in animal models of cryptococcosis. Consistent with its predicted function to inhibit and sequester Rho-type GTPases, overexpression of\n                    <jats:italic>RDI1<\/jats:italic>\n                    results in cytosolic localization of Cdc42. Likely as a result of this finding,\n                    <jats:italic>RDI1<\/jats:italic>\n                    -overexpressing strains exhibited altered morphology compared to that of the wild type, with apparent defects in maintaining proper cell polarity and cytokinesis.\n                    <jats:italic>RDI1<\/jats:italic>\n                    deletion resulted in increased vacuole size in tissue culture medium and aberrant cell morphology at neutral pH. Maintenance of normal cell morphology is vital for\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    pathogenicity. Accordingly, the\n                    <jats:italic>rdi1<\/jats:italic>\n                    \u0394 mutant strain also showed reduced intracellular survival in macrophages and severe attenuation of virulence in two murine models of cryptococcosis. This reduction in virulence of the\n                    <jats:italic>rdi1<\/jats:italic>\n                    \u0394 mutant occurs in the absence of major growth defects in rich medium and with classical virulence-associated phenotypes.\n                  <\/jats:p>","DOI":"10.1128\/iai.00896-08","type":"journal-article","created":{"date-parts":[[2008,9,8]],"date-time":"2008-09-08T20:33:56Z","timestamp":1220906036000},"page":"5729-5737","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["The\n                    <i>Cryptococcus neoformans<\/i>\n                    Rho-GDP Dissociation Inhibitor Mediates Intracellular Survival and Virulence"],"prefix":"10.1128","volume":"76","author":[{"given":"Michael S.","family":"Price","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology"},{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie B.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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