{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:24:52Z","timestamp":1792599892238,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Both decorin-binding proteins (DbpA and DbpB) of the Lyme disease spirochete\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            bind decorin and glycosaminoglycans, two important building blocks of proteoglycans that are abundantly found in the extracellular matrix (ECM) and connective tissues as well as on cell surfaces of mammals. As an extracellular pathogen,\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            resides primarily in the ECM and connective tissues and between host cells during mammalian infection. The interactions of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            with these host ligands mediated by DbpA and DbpB potentially influence various aspects of infection. Here, we show that both DbpA and DbpB are critical for the overall virulence of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            in the murine host. Disruption of the\n            <jats:italic>dbpBA<\/jats:italic>\n            locus led to nearly a 10\n            <jats:sup>4<\/jats:sup>\n            -fold increase in the 50% infectious dose (ID\n            <jats:sub>50<\/jats:sub>\n            ). Complementation of the mutant with either\n            <jats:italic>dbpA<\/jats:italic>\n            or\n            <jats:italic>dbpB<\/jats:italic>\n            reduced the ID\n            <jats:sub>50<\/jats:sub>\n            from over 10\n            <jats:sup>4<\/jats:sup>\n            to roughly 10\n            <jats:sup>3<\/jats:sup>\n            organisms. Deletion of the\n            <jats:italic>dbpBA<\/jats:italic>\n            locus affected colonization in all tissues of infected mice. The lack of\n            <jats:italic>dbpA<\/jats:italic>\n            alone precluded the pathogen from colonizing the heart tissue, and\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            deficient for DbpB was recovered only from 42% of the heart specimens of infected mice. Although\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            lacking either\n            <jats:italic>dbpA<\/jats:italic>\n            or\n            <jats:italic>dbpB<\/jats:italic>\n            was consistently grown from joint specimens of almost all infected mice, it generated bacterial loads significantly lower than the control. The deficiency in either DbpA or DbpB did not reduce the bacterial load in skin, but lack of both significantly did. Taken together, the study results indicate that neither DbpA nor DbpB is essential for mammalian infection but that both are critical for the overall virulence of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00897-07","type":"journal-article","created":{"date-parts":[[2008,1,15]],"date-time":"2008-01-15T01:34:16Z","timestamp":1200360856000},"page":"1239-1246","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Both Decorin-Binding Proteins A and B Are Critical for the Overall Virulence of\n            <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Yanlin","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qilong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristy","family":"McShan","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang Ting","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.2.419"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.657"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26272"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11692"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb31851.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2007.06.003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01654.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04759.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3455-3459.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1159\/000073406"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.435-441.2006"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1231043100"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01103.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.6.2674-2683.1998"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4759-4764.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05746.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7147-7154.2004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4608-4613.2003"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4798-4804.2002"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02035-05"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63359-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.3016-3020.2006"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01792.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1743-1749.1999"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.01127.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4129-4133.2001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03452.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.25.13865"},{"key":"e_1_3_2_31_2","first-page":"493","volume":"27","year":"1938","unstructured":"Reed, L. J., and H. Muench. 1938. A simple method of estimating fifty per cent endpoint. Am. J. Hyg.27:493-497.","journal-title":"Am. J. Hyg."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(96)80038-0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05042.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00740-06"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200107123450207"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00459-06"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05636.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7208-7215.2005"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.8.5109"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00560-07"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00897-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00897-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:31:58Z","timestamp":1646465518000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00897-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1128\/IAI.00897-07"],"URL":"https:\/\/doi.org\/10.1128\/iai.00897-07","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3]]},"assertion":[{"value":"2007-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}