{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:58:56Z","timestamp":1791298736774,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , the etiological agent of Legionnaires disease, is known to trigger pore formation in bone marrow-derived macrophages (BMMs) by mechanisms dependent on the type IVB secretion system known as Dot\/Icm. Here, we used several mutants of\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    in combination with knockout mice to assess the host and bacterial factors involved in pore formation in BMMs. We found that regardless of Dot\/Icm activity, pore formation does not occur in BMMs deficient in caspase-1 and Nlrc4\/Ipaf. Pore formation was temporally associated with interleukin-1\u03b2 secretion and preceded host cell lysis and pyroptosis. Pore-forming ability was dependent on bacterial Dot\/Icm but independent of several effector proteins, multiplication, and\n                    <jats:italic>de novo<\/jats:italic>\n                    protein synthesis. Flagellin, which is known to trigger the Nlrc4 inflammasome, was required for pore formation as\n                    <jats:italic>flaA<\/jats:italic>\n                    mutant bacteria failed to induce cell permeabilization. Accordingly, transfection of purified flagellin was sufficient to trigger pore formation independent of infection. By using 11 different\n                    <jats:italic>Legionella<\/jats:italic>\n                    species, we found robust pore formation in response to\n                    <jats:italic>L. micdadei<\/jats:italic>\n                    ,\n                    <jats:italic>L. bozemanii<\/jats:italic>\n                    ,\n                    <jats:italic>L. gratiana<\/jats:italic>\n                    ,\n                    <jats:italic>L. jordanis<\/jats:italic>\n                    , and\n                    <jats:italic>L. rubrilucens<\/jats:italic>\n                    , and this trait correlated with flagellin expression by these species. Together, the results suggest that pore formation is neither\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    specific nor the result of membrane damage induced by Dot\/Icm activity; instead, it is a highly coordinated host cell response dependent on host Nlrc4 and caspase-1 and on bacterial flagellin and type IV secretion system.\n                  <\/jats:p>","DOI":"10.1128\/iai.00905-09","type":"journal-article","created":{"date-parts":[[2010,1,4]],"date-time":"2010-01-04T21:10:40Z","timestamp":1262639440000},"page":"1403-1413","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":95,"title":["Pore Formation Triggered by\n                    <i>Legionella<\/i>\n                    spp. Is an Nlrc4 Inflammasome-Dependent Host Cell Response That Precedes Pyroptosis"],"prefix":"10.1128","volume":"78","author":[{"given":"Tatiana N.","family":"Silveira","sequence":"first","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeira\u0303o Preto, University of Sa\u0303o Paulo, Ribeira\u0303o Preto, SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario S.","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine of Ribeira\u0303o Preto, University of Sa\u0303o Paulo, Ribeira\u0303o Preto, SP 14049-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.3021-3026.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000361"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.4.1351-1363.2006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.11.6431-6440.2000"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604933200"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.950-958.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01092.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.2927-2938.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030188"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01382-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000501"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.9.2912-2918.1990"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02176.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/15687"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e3282f79678"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020034"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.33.12.2132"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2008.11.007"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.004"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.10.4.437-441.1979"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00751.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01036.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01546-08"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1346"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1967"},{"key":"e_1_3_2_28_2","first-page":"220","volume":"2","year":"1993","unstructured":"Isberg, R. 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