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In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of<jats:italic>L. pneumophila<\/jats:italic>. The<jats:italic>ankH<\/jats:italic>and<jats:italic>ankJ<\/jats:italic>mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the<jats:italic>ankH<\/jats:italic>or<jats:italic>ankJ<\/jats:italic>mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot\/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell.<\/jats:p>","DOI":"10.1128\/iai.00913-09","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T02:10:09Z","timestamp":1261534209000},"page":"1123-1134","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Molecular Characterization of the Dot\/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of<i>Legionella pneumophila<\/i>"],"prefix":"10.1128","volume":"78","author":[{"given":"Fabien","family":"Habyarimana","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris T.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Santic","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souhaila","family":"Al-Khodor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef Abu","family":"Kwaik","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Cell Microbiol. 2007"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3127-3133.1998"},{"key":"e_1_3_1_4_2","unstructured":"Trends Microbiol."},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1111\/j.1365-2958.2008.06453.x","volume":"70","year":"2008","unstructured":"Al-Khodor, S., C. 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