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Survival under these prolonged nutrient-limited conditions is thought to be similar to survival during stationary phase, which is characterized by growth cessation and decreased protein production. Multiple ribosome-associated proteins are implicated in stationary-phase survival of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            . These proteins include hibernation-promoting factor (HPF), which dimerizes ribosomes and prevents translation of mRNA. Bioinformatic analyses indicate that\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            harbors an\n            <jats:italic>hpf<\/jats:italic>\n            homolog, the\n            <jats:italic>bb0449<\/jats:italic>\n            gene. BB0449 protein secondary structure modeling also predicted HPF-like structure and function. However, BB0449 protein was not localized in the ribosome-associated protein fraction of\n            <jats:italic>in vitro<\/jats:italic>\n            -grown\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            . In wild-type\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            ,\n            <jats:italic>bb0449<\/jats:italic>\n            transcript and BB0449 protein levels are low during various growth phases. These results are inconsistent with patterns of synthesis of HPF-like proteins in other bacterial species. In addition, two independently derived\n            <jats:italic>bb0449<\/jats:italic>\n            mutants successfully completed the mouse-tick infectious cycle, indicating that\n            <jats:italic>bb0449<\/jats:italic>\n            is not required for prolonged survival in the nutrient-limited environment in the unfed tick or any other stage of infection by\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            . We suggest either that BB0449 is associated with ribosomes under specific conditions not yet identified or that BB0449 of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            has a function other than ribosome conformation modulation.\n          <\/jats:p>","DOI":"10.1128\/iai.00925-15","type":"journal-article","created":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T00:11:36Z","timestamp":1444090296000},"page":"4800-4810","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Long-Term Survival of Borrelia burgdorferi Lacking the Hibernation Promotion Factor Homolog in the Unfed Tick Vector"],"prefix":"10.1128","volume":"83","author":[{"given":"Lisa","family":"Fazzino","sequence":"first","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kit","family":"Tilly","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel P.","family":"Dulebohn","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1","article-title":"Surveillance for Lyme disease\u2014United States, 1992\u20132006","volume":"57","author":"Bacon RM","year":"2008","unstructured":"Bacon RM, Kugeler KJ, Mead PS. 2008. 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