{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T17:50:35Z","timestamp":1781286635704,"version":"3.54.1"},"reference-count":75,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["PNPD"],"award-info":[{"award-number":["PNPD"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/07237-1"],"award-info":[{"award-number":["2015\/07237-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/51832-3"],"award-info":[{"award-number":["2012\/51832-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/12940-3"],"award-info":[{"award-number":["2015\/12940-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Streptococcus sanguinis<\/jats:named-content>\n is a pioneer species of teeth and a common opportunistic pathogen of infective endocarditis. In this study, we identified a two-component system,\n S. sanguinis<\/jats:named-content>\n SptRS (SptRS\n \n Ss<\/jats:italic>\n <\/jats:sub>\n ), affecting\n S. sanguinis<\/jats:named-content>\n survival in saliva and biofilm formation. Isogenic mutants of\n \n sptR\n Ss<\/jats:sub>\n <\/jats:italic>\n (SKsptR) and\n \n sptS\n Ss<\/jats:sub>\n <\/jats:italic>\n (SKsptS) showed reduced cell counts in\n ex vivo<\/jats:italic>\n assays of viability in saliva compared to those of parent strain SK36 and complemented mutants. Reduced counts of the mutants in saliva were associated with reduced growth rates in nutrient-poor medium (RPMI) and increased susceptibility to the deposition of C3b and the membrane attach complex (MAC) of the complement system, a defense component of saliva and serum. Conversely,\n \n sptR\n Ss<\/jats:sub>\n <\/jats:italic>\n and\n \n sptS\n Ss<\/jats:sub>\n <\/jats:italic>\n mutants showed increased biofilm formation associated with higher levels of production of H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n and extracellular DNA. Reverse transcription-quantitative PCR (RT-qPCR) comparisons of strains indicated a global role of SptRS\n \n Ss<\/jats:italic>\n <\/jats:sub>\n in repressing genes for H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n production (2.5- to 15-fold upregulation of\n spxB<\/jats:italic>\n ,\n spxR<\/jats:italic>\n ,\n vicR<\/jats:italic>\n ,\n tpk<\/jats:italic>\n , and\n ackA<\/jats:italic>\n in\n \n sptR\n Ss<\/jats:sub>\n <\/jats:italic>\n and\n \n sptS\n Ss<\/jats:sub>\n <\/jats:italic>\n mutants), biofilm formation, and\/or evasion of host immunity (2.1- to 11.4-fold upregulation of\n srtA<\/jats:italic>\n ,\n pcsB<\/jats:italic>\n ,\n cwdP<\/jats:italic>\n ,\n iga<\/jats:italic>\n , and\n nt5e<\/jats:italic>\n ). Compatible with the homology of SptR\n \n Ss<\/jats:italic>\n <\/jats:sub>\n with AraC-type regulators, duplicate to multiple conserved repeats were identified in 1,000-bp regulatory regions of downstream genes, suggesting that SptR\n \n Ss<\/jats:italic>\n <\/jats:sub>\n regulates transcription by DNA looping. Significant transcriptional changes in the regulatory genes\n vicR<\/jats:italic>\n ,\n spxR<\/jats:italic>\n ,\n comE<\/jats:italic>\n ,\n comX<\/jats:italic>\n , and\n mecA<\/jats:italic>\n in the\n \n sptR\n Ss<\/jats:sub>\n <\/jats:italic>\n and\n \n sptS\n Ss<\/jats:sub>\n <\/jats:italic>\n mutants further indicated that SptRS\n \n Ss<\/jats:italic>\n <\/jats:sub>\n is part of a regulatory network that coordinates cell wall homeostasis, H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n production, and competence. This study reveals that SptRS\n \n Ss<\/jats:italic>\n <\/jats:sub>\n is involved in the regulation of crucial functions for\n S. sanguinis<\/jats:named-content>\n persistence in the oral cavity.\n <\/jats:p>","DOI":"10.1128\/iai.00942-17","type":"journal-article","created":{"date-parts":[[2018,1,11]],"date-time":"2018-01-11T10:59:40Z","timestamp":1515668380000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Novel Two-Component System of Streptococcus sanguinis Affecting Functions Associated with Viability in Saliva and Biofilm Formation"],"prefix":"10.1128","volume":"86","author":[{"given":"Tarsila M.","family":"Camargo","sequence":"first","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael N.","family":"Stipp","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia A.","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika N.","family":"Harth-Chu","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 F.","family":"H\u00f6fling","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata O.","family":"Mattos-Graner","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(70)90005-1"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(99)90074-9"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2005.085332"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-39-3-179"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4018-4023.2000"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.21.7193-7203.2005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00014-09"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067919"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00906-09"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00276-08"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01850-14"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.183"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073214"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150039"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505839102"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027320425291"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13044295"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05897.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MBP-0013-2014"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12170"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01429.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12244"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200104053441406"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12170"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12461"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09643.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.06082.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.039669-0"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-183"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038059"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.10.3087-3095.1999"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00052.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058271"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.2.476"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90435-2"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.3.371-394.1991"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.4.393-410.1997"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8703(99)70294-2"},{"key":"e_1_3_3_40_2","article-title":"Characterization of the SptR regulon of Streptococcus mutans, abstr 126","author":"Vizoto NL","year":"2014","unstructured":"Vizoto NL, Scott JC, Boisvert H, Duncan MJ, Mattos-Graner RO. 2014. Characterization of the SptR regulon of Streptococcus mutans, abstr 126. Abstr 114th Gen Meet Am Soc Microbiol.","journal-title":"Abstr 114th Gen Meet Am Soc Microbiol"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4723-4731.2005"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515611074"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916642614"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.03.001"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01808-06"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.610212"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.178350"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.09.018"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.04.025"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.08.010"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00760-09"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1991.tb00466.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(83)90097-3"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.52.2.296-303.1988"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00303-12"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.1.31-39.2000"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.39"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.177.4050.697"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2009.03.013"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2006.00272.x"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.3990-3997.2000"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01131-10"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13016"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03714.x"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2006.06.007"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026403"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(82)90025-7"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.4.1194-1201.1991"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00369-4"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660021901"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-16"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2008.00451.x"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.5857"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku949"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1129"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn304"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00942-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00942-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:51:35Z","timestamp":1646477495000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00942-17"}},"subtitle":[],"editor":[{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":75,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1128\/IAI.00942-17"],"URL":"https:\/\/doi.org\/10.1128\/iai.00942-17","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"2017-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00942-17"}}