{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:30:28Z","timestamp":1764783028592},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An experimental DNA plasmid vaccine was developed based on a well-characterized and protective peptide epitope derived from a bacterial porin protein. For this study, we used the P1.16b serosubtype epitope, located in variable region (VR)2 in loop 4 of the PorA outer membrane (OM) porin from\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            serogroup B strain MC58. A plasmid that encoded the entire loop (pPorAloop4) was prepared, as well as a fusion plasmid that encoded the loop in tandem with the fragment C (FrC) immunostimulatory sequence from tetanus toxin (pPorAloop4-FrC). The constructs were used for intramuscular immunization without exogenous adjuvant. Murine antisera raised to the pPorAloop4-FrC DNA fusion plasmid reacted significantly with OMs in enzyme-linked immunosorbent assay and with whole bacteria by immunofluorescence, whereas antisera raised to the pPorAloop4 DNA plasmid and to control plasmid showed little or no reactivity. Significantly, only the pPorALoop4-FrC plasmid induced bactericidal antibodies, demonstrating that the intrinsic immunostimulatory sequence was essential for inducing a protective immune response. The antibodies raised to the P1.16b pPorALoop4-FrC plasmid were serosubtype specific, showing no significant immunofluorescence reactivity or bactericidal activity against other PorA variants. These data provide proof of principle for a DNA fusion plasmid strategy as a novel approach to preparing vaccines based on defined, protective epitopes.\n          <\/jats:p>","DOI":"10.1128\/iai.00943-07","type":"journal-article","created":{"date-parts":[[2007,10,29]],"date-time":"2007-10-29T23:00:08Z","timestamp":1193698808000},"page":"334-338","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["A DNA Fusion Vaccine Induces Bactericidal Antibodies to a Peptide Epitope from the PorA Porin of\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Delin","family":"Zhu","sequence":"first","affiliation":[{"name":"Genetic Vaccine Group, Cancer Sciences Division, Somers Cancer Research Building"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannette N.","family":"Williams","sequence":"additional","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Rice","sequence":"additional","affiliation":[{"name":"Genetic Vaccine Group, Cancer Sciences Division, Somers Cancer Research Building"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Freda K.","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Genetic Vaccine Group, Cancer Sciences Division, Somers Cancer Research Building"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E.","family":"Heckels","sequence":"additional","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myron","family":"Christodoulides","sequence":"additional","affiliation":[{"name":"Neisseria Research Group, Molecular Microbiology, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051540"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.08.027"},{"key":"e_1_3_2_4_2","first-page":"97","volume":"121","year":"2005","unstructured":"Biering, E., S. 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