{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:10:20Z","timestamp":1791400220387,"version":"4.3.3"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Choloylglycine hydrolase (CGH), a bile salt hydrolase, has been annotated in all the available genomes of\n            <jats:italic>Brucella<\/jats:italic>\n            species. We obtained the\n            <jats:italic>Brucella<\/jats:italic>\n            CGH in recombinant form and demonstrated in vitro its capacity to cleave glycocholate into glycine and cholate.\n            <jats:italic>Brucella abortus<\/jats:italic>\n            2308 (wild type) and its isogenic \u0394\n            <jats:italic>cgh<\/jats:italic>\n            deletion mutant exhibited similar growth rates in tryptic soy broth in the absence of bile. In contrast, the growth of the \u0394\n            <jats:italic>cgh<\/jats:italic>\n            mutant was notably impaired by both 5% and 10% bile. The bile resistance of the complemented mutant was similar to that of the wild-type strain. In mice infected through the intragastric or the intraperitoneal route, splenic infection was significantly lower at 10 and 20 days postinfection in animals infected with the \u0394\n            <jats:italic>cgh<\/jats:italic>\n            mutant than in those infected with the wild-type strain. For both routes, no differences in spleen CFU were found between animals infected with the wild-type strain and those infected with the complemented mutant. Mice immunized intragastrically with recombinant CGH mixed with cholera toxin (CGH+CT) developed a specific mucosal humoral (immunoglobulin G [IgG] and IgA) and cellular (interleukin-2) immune responses. Fifteen days after challenge by the same route with live\n            <jats:italic>B. abortus<\/jats:italic>\n            2308 cells, splenic CFU counts were 10-fold lower in mice immunized with CGH+CT than in mice immunized with CT or phosphate-buffered saline. This study shows that CGH confers on\n            <jats:italic>Brucella<\/jats:italic>\n            the ability to resist the antimicrobial action of bile salts. The results also suggest that CGH may contribute to the ability of\n            <jats:italic>Brucella<\/jats:italic>\n            to infect the host through the oral route.\n          <\/jats:p>","DOI":"10.1128\/iai.00952-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"299-305","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["A Bile Salt Hydrolase of\n            <i>Brucella abortus<\/i>\n            Contributes to the Establishment of a Successful Infection through the Oral Route in Mice"],"prefix":"10.1128","volume":"75","author":[{"given":"M. Victoria","family":"Delpino","sequence":"first","affiliation":[{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU, CONICET-UBA), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a I.","family":"Marchesini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de Gral. San Marti\u0301n, 1650 San Marti\u0301n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia M.","family":"Estein","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunologi\u0301a, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, 7000 Tandil, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de Gral. San Marti\u0301n, 1650 San Marti\u0301n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Cassataro","sequence":"additional","affiliation":[{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU, CONICET-UBA), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina"},{"name":"Laboratorio de Inmunogene\u0301tica, Hospital de Cli\u0301nicas \u201cJose\u0301 de San Marti\u0301n,\u201d Facultad de Medicina, Universidad de Buenos Aires, 1120 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Fossati","sequence":"additional","affiliation":[{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU, CONICET-UBA), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo C.","family":"Baldi","sequence":"additional","affiliation":[{"name":"Instituto de Estudios de la Inmunidad Humoral (IDEHU, CONICET-UBA), Facultad de Farmacia y Bioqui\u0301mica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8353-8361.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1995.tb03140.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03080.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)38464-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2000.01147.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600780103"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.9.5603-5612.2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.8.4171-4177.1990"},{"key":"e_1_3_2_12_2","first-page":"41","year":"1989","unstructured":"Nicoletti, P. L. 1989. Relationship between animal and human disease, p. 41-51. In E. J. Young and M. J. Corbel (ed.), Brucellosis: clinical and laboratory aspects, CRC Press, Boca Raton, FL.","journal-title":"Brucellosis: clinical and laboratory aspects"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192319099"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0473206"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02777700"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(76)90068-1"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2502-2512.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.12.2772"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00952-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00952-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:59:48Z","timestamp":1646463588000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00952-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1128\/IAI.00952-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.00952-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]},"assertion":[{"value":"2006-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-10-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}