{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:35:07Z","timestamp":1791095707043,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Rickettsia rickettsii<\/jats:italic>\n is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. To identify genes involved in the virulence of\n R. rickettsii<\/jats:italic>\n , the genome of an avirulent strain,\n R. rickettsii<\/jats:italic>\n Iowa, was sequenced and compared to the genome of the virulent strain\n R. rickettsii<\/jats:italic>\n Sheila Smith.\n R. rickettsii<\/jats:italic>\n Iowa is avirulent in a guinea pig model of infection and displays altered plaque morphology with decreased lysis of infected host cells. Comparison of the two genomes revealed that\n R. rickettsii<\/jats:italic>\n Iowa and\n R. rickettsii<\/jats:italic>\n Sheila Smith share a high degree of sequence identity. A whole-genome alignment comparing\n R. rickettsii<\/jats:italic>\n Iowa to\n R. rickettsii<\/jats:italic>\n Sheila Smith revealed a total of 143 deletions for the two strains. A subsequent single-nucleotide polymorphism (SNP) analysis comparing Iowa to Sheila Smith revealed 492 SNPs for the two genomes. One of the deletions in\n R. rickettsii<\/jats:italic>\n Iowa truncates\n rompA<\/jats:italic>\n , encoding a major surface antigen (rickettsial outer membrane protein A [rOmpA]) and member of the autotransporter family, 660 bp from the start of translation. Immunoblotting and immunofluorescence confirmed the absence of rOmpA from\n R. rickettsii<\/jats:italic>\n Iowa. In addition,\n R. rickettsii<\/jats:italic>\n Iowa is defective in the processing of rOmpB, an autotransporter and also a major surface antigen of spotted fever group rickettsiae. Disruption of\n rompA<\/jats:italic>\n and the defect in rOmpB processing are most likely factors that contribute to the avirulence of\n R. rickettsii<\/jats:italic>\n Iowa. Genomic differences between the two strains do not significantly alter gene expression as analysis of microarrays revealed only four differences in gene expression between\n R. rickettsii<\/jats:italic>\n Iowa and\n R. rickettsii<\/jats:italic>\n strain R. Although\n R. rickettsii<\/jats:italic>\n Iowa does not cause apparent disease, infection of guinea pigs with this strain confers protection against subsequent challenge with the virulent strain\n R. rickettsii<\/jats:italic>\n Sheila Smith.\n <\/jats:p>","DOI":"10.1128\/iai.00952-07","type":"journal-article","created":{"date-parts":[[2007,11,19]],"date-time":"2007-11-19T21:08:18Z","timestamp":1195506498000},"page":"542-550","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Genomic Comparison of Virulent\n Rickettsia rickettsii<\/i>\n Sheila Smith and Avirulent\n Rickettsia rickettsii<\/i>\n Iowa"],"prefix":"10.1128","volume":"76","author":[{"given":"Damon W.","family":"Ellison","sequence":"first","affiliation":[{"name":"Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina R.","family":"Clark","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Sturdevant","sequence":"additional","affiliation":[{"name":"Genomics Unit, Research Technology Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimmo","family":"Virtaneva","sequence":"additional","affiliation":[{"name":"Genomics Unit, Research Technology Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen F.","family":"Porcella","sequence":"additional","affiliation":[{"name":"Genomics Unit, Research Technology Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ted","family":"Hackstadt","sequence":"additional","affiliation":[{"name":"Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.2.653-660.1986"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.1.167-171.1987"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.27.2.468-474.1980"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.3.559-564.1984"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01312-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/24094"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.11.6628-6636.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.94.2444.399"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221575398"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.4.788-796.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07449.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.2.556-565.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000266"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4439(91)90027-7"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02318"},{"key":"e_1_3_2_18_2","first-page":"127","volume":"5","year":"1996","unstructured":"Hackstadt, T. 1996. 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