{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:00:16Z","timestamp":1792670416037,"version":"4.5.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Key features of\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            K1-mediated neonatal sepsis and meningitis, such as a strong age dependency and development along the gut-mesentery-blood-brain course of infection, can be replicated in the newborn rat. We examined temporal and spatial aspects of\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            K1 infection following initiation of gastrointestinal colonization in 2-day-old (P2) rats after oral administration of\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            K1 strain A192PP and a virulent bioluminescent derivative,\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            A192PP-\n            <jats:italic>lux<\/jats:italic>\n            2. A combination of bacterial enumeration in the major organs, two-dimensional bioluminescence imaging, and three-dimensional diffuse light imaging tomography with integrated micro-computed tomography indicated multiple sites of colonization within the alimentary canal; these included the tongue, esophagus, and stomach in addition to the small intestine and colon. After invasion of the blood compartment, the bacteria entered the central nervous system, with restricted colonization of the brain, and also invaded the major organs, in line with increases in the severity of symptoms of infection. Both keratinized and nonkeratinized surfaces of esophagi were colonized to a considerably greater extent in susceptible P2 neonates than in corresponding tissues from infection-resistant 9-day-old rat pups; the bacteria appeared to damage and penetrate the nonkeratinized esophageal epithelium of infection-susceptible P2 animals, suggesting the esophagus represents a portal of entry for\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            K1 into the systemic circulation. Thus, multimodality imaging of experimental systemic infections in real time indicates complex dynamic patterns of colonization and dissemination that provide new insights into the\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            K1 infection of the neonatal rat.\n          <\/jats:p>","DOI":"10.1128\/iai.00953-15","type":"journal-article","created":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T02:15:51Z","timestamp":1441764951000},"page":"4528-4540","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Bioluminescent Imaging Reveals Novel Patterns of Colonization and Invasion in Systemic Escherichia coli K1 Experimental Infection in the Neonatal Rat"],"prefix":"10.1128","volume":"83","author":[{"given":"Luci A.","family":"Witcomb","sequence":"first","affiliation":[{"name":"University College London School of Pharmacy, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Collins","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex J.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"University College London School of Pharmacy, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gadi","family":"Frankel","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"University College London School of Pharmacy, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.118"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.011"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-13"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)92496-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197602000-00002"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-9-2745"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050177"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.26906"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007-2171"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00070-09"},{"key":"e_1_3_3_12_2","first-page":"e52018","article-title":"Non-invasive model of neuropathogenic Escherichia coli infection in the neonatal rat","volume":"92","author":"Dalgakiran F","year":"2014","unstructured":"Dalgakiran F, Witcomb LA, McCarthy AJ, Birchenough GM, Taylor PW. 2014. 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