{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:15:52Z","timestamp":1771953352807,"version":"3.50.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Coccidioidomycosis is a human respiratory disease that is endemic to the southwestern United States and is caused by inhalation of the spores of a desert soilborne fungus. Efforts to develop a vaccine against this disease have focused on identification of T-cell-reactive antigens derived from the parasitic cell wall which can stimulate protective immunity against<jats:italic>Coccidioides posadasii<\/jats:italic>infection in mice. We previously described a productive immunoproteomic\/bioinformatic approach to the discovery of vaccine candidates which makes use of the translated genome of<jats:italic>C. posadasii<\/jats:italic>and a computer-based method of scanning deduced sequences of seroreactive proteins for epitopes that are predicted to bind to human major histocompatibility (MHC) class II-restricted molecules. In this study we identified a set of putative cell wall proteins predicted to contain multiple, promiscuous MHC II binding epitopes. Three of these were expressed by<jats:italic>Escherichia coli<\/jats:italic>, combined in a vaccine, and tested for protective efficacy in C57BL\/6 mice. Approximately 90% of the mice survived beyond 90 days after intranasal challenge, and the majority cleared the pathogen. We suggest that the multicomponent vaccine stimulates a broader range of T-cell clones than the single recombinant protein vaccines and thereby may be capable of inducing protection in an immunologically heterogeneous human population.<\/jats:p>","DOI":"10.1128\/iai.00961-06","type":"journal-article","created":{"date-parts":[[2006,9,20]],"date-time":"2006-09-20T23:06:35Z","timestamp":1158793595000},"page":"5802-5813","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Multivalent Recombinant Protein Vaccine against Coccidioidomycosis"],"prefix":"10.1128","volume":"74","author":[{"given":"Eric J.","family":"Tarcha","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkatesha","family":"Basrur","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, Ohio 43614"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiung-Yu","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm J.","family":"Gardner","sequence":"additional","affiliation":[{"name":"Seattle Biomedical Research Institute, Seattle, Washington 98109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Garry T.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, Texas 78249"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.6.3900"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0705.010505"},{"key":"e_1_3_2_5_2","first-page":"1","volume":"52","year":"2005","unstructured":"Centers for Disease Control and Prevention. 2005. Summary of notifiable diseases\u2014United States, 2003. Morb. Mortal. Wkly. Rep.52:1-88.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780410001687349"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7786"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0933"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01409-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.01.059"},{"key":"e_1_3_2_11_2","first-page":"52","volume":"36","year":"1998","unstructured":"De Hoog, G. S., B. Bowman, Y. Graser, G. Haase, M. El Fari, A. H. Gerrits van den Ende, B. Melzer-Krick, and W. A. Untereiner. 1998. Molecular phylogeny and taxonomy of medically important fungi. Med. Mycol.36(Suppl. 1):52-56.","journal-title":"Med. Mycol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3010-3019.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.4.2415-2422.2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/503043"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(97)01040-5"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.3.372"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti299"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/502972"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1A410"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.584-593.2000"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6689-6703.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2211-2222.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3443-3456.2002"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.6.2635"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/497596"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwh151"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.092"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)02038-5"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.11.7496"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.2878-2887.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/15525"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.504-515.2006"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00223"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1865-1872.2006"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00654-0"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80111-3"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.04.002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1236"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.4049\/jimmunol.160.7.3363","volume":"160","year":"1998","unstructured":"Southwood, S., J. Sidney, A. Kondo, M.-F. del Guercio, E. Appella, S. Hoffman, R. T. Kubo, R. W. Chesnut, H. M. Grey, and A. Sette. 1998. Several common HLA-DR types share largely overlapping peptide binding repertoires. J. Immunol.160:3363-3373.","journal-title":"J. Immunol."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.6.1672"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.516-527.2006"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5462-5469.2005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00961-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00961-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T22:50:36Z","timestamp":1683586236000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00961-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":41,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1128\/IAI.00961-06"],"URL":"https:\/\/doi.org\/10.1128\/iai.00961-06","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]},"assertion":[{"value":"2006-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}