{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:32:47Z","timestamp":1787016767640,"version":"build-2736575974"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["1R21AI119634"],"award-info":[{"award-number":["1R21AI119634"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007165","name":"University of Missouri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007165","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    spp. are facultative intracellular Gram-negative bacteria that cause the zoonotic disease brucellosis, one of the most common global zoonoses. Osteomyelitis, arthritis, and musculoskeletal inflammation are common focal complications of brucellosis in humans; however, wild-type (WT) mice infected systemically with conventional doses of\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    do not develop these complications. Here we report C57BL\/6 WT mice infected via the footpad with 10\n                    <jats:sup>3<\/jats:sup>\n                    to 10\n                    <jats:sup>6<\/jats:sup>\n                    CFU of\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    spp. display neutrophil and monocyte infiltration of the joint space and surrounding musculoskeletal tissue. Joint inflammation is detectable as early as 1 day postinfection and peaks 1 to 2 weeks later, after which WT mice are able to slowly resolve inflammation. B and T cells were dispensable for the onset of swelling but required for resolution of joint inflammation and infection. At early time points, MyD88\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice display decreased joint inflammation, swelling, and proinflammatory cytokine levels relative to WT mice. Subsequently, swelling of MyD88\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    joints surpassed WT joint swelling, and resolution of joint inflammation was prolonged. Joint bacterial loads in MyD88\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were significantly greater than those in WT mice by day 3 postinfection and at all time points thereafter. In addition, MyD88\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    joint inflammatory cytokine levels on day 3 and beyond were similar to WT levels. Collectively these data demonstrate MyD88 signaling mediates early inflammatory responses in the joint but also contributes to subsequent clearance of\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    and resolution of inflammation. This work also establishes a mouse model for studying\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    -induced arthritis, musculoskeletal complications, and systemic responses, which will lead to a better understanding of focal complications of brucellosis.\n                  <\/jats:p>","DOI":"10.1128\/iai.00961-16","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T21:20:42Z","timestamp":1483996842000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Temporal Role for MyD88 in a Model of Brucella-Induced Arthritis and Musculoskeletal Inflammation"],"prefix":"10.1128","volume":"85","author":[{"given":"Carolyn A.","family":"Lacey","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerod A.","family":"Skyberg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.50.1.23"},{"key":"e_1_3_2_3_2","first-page":"ix","article-title":"Research priorities for zoonoses and marginalized infections","volume":"2012","year":"2012","unstructured":". 2012. 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