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One of the best known pathogenicity factors of<jats:italic>B. henselae<\/jats:italic>is<jats:italic>Bartonella<\/jats:italic>adhesin A (BadA), which is modularly constructed, consisting of head, neck\/stalk, and membrane anchor domains. BadA is important for the adhesion of<jats:italic>B. henselae<\/jats:italic>to extracellular-matrix proteins and endothelial cells (ECs). In this study, we analyzed different<jats:italic>B. henselae<\/jats:italic>strains for BadA expression, autoagglutination, fibronectin (Fn) binding, and adhesion to ECs. We found that the<jats:italic>B. henselae<\/jats:italic>strains Marseille, ATCC 49882, Freiburg 96BK3 (FR96BK3), FR96BK38, and G-5436 express BadA. Remarkably, BadA expression was lacking in a<jats:italic>B. henselae<\/jats:italic>ATCC 49882 variant, in strains ATCC 49793 and Berlin-1, and in the majority of bacteria of strain Berlin-2. Adherence of<jats:italic>B. henselae<\/jats:italic>to ECs and Fn reliably correlated with BadA expression.<jats:italic>badA<\/jats:italic>was present in all tested strains, although the length of the gene varied significantly due to length variations of the stalk region. Sequencing of the promoter, head, and membrane anchor regions revealed only minor differences that did not correlate with BadA expression, apart from strain Berlin-1, in which a 1-bp deletion led to a frameshift in the head region of BadA. Our data suggest that, apart from the identified genetic modifications (frameshift deletion and recombination), other so-far-unknown regulatory mechanisms influence BadA expression. Because of variations between and within different<jats:italic>B. henselae<\/jats:italic>isolates, BadA expression should be analyzed before performing infection experiments with<jats:italic>B. henselae.<\/jats:italic><\/jats:p>","DOI":"10.1128\/iai.00963-06","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:51:25Z","timestamp":1166658685000},"page":"35-43","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Analysis of<i>Bartonella<\/i>Adhesin A Expression Reveals Differences between Various<i>B. henselae<\/i>Strains"],"prefix":"10.1128","volume":"75","author":[{"given":"Tanja","family":"Riess","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universita\u0308t, Elfriede-Aulhorn-Str. 6, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gu\u0308nter","family":"Raddatz","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r biologische Kybernetik, Spemannstr. 34, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Linke","sequence":"additional","affiliation":[{"name":"Max-Planck-Institut fu\u0308r Entwicklungsbiologie, Abteilung Proteinevolution, Spemannstr. 35, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Scha\u0308fer","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universita\u0308t, Elfriede-Aulhorn-Str. 6, 72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volkhard A. 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