{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:32:53Z","timestamp":1787740373455,"version":"build-2784847793"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI099122"],"award-info":[{"award-number":["R01AI099122"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI112694"],"award-info":[{"award-number":["R21AI112694"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI076691"],"award-info":[{"award-number":["R21AI076691"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n We established a new\n Brucella neotomae<\/jats:named-content>\n in vitro<\/jats:italic>\n model system for study of type IV secretion system-dependent (T4SS) pathogenesis in the\n Brucella<\/jats:named-content>\n genus. Importantly,\n B. neotomae<\/jats:named-content>\n is a rodent pathogen, and unlike\n B. abortus<\/jats:named-content>\n ,\n B. melitensis<\/jats:named-content>\n , and\n B. suis<\/jats:named-content>\n ,\n B. neotomae<\/jats:named-content>\n has not been observed to infect humans. It therefore can be handled more facilely using biosafety level 2 practices. More particularly, using a series of novel fluorescent protein and\n lux<\/jats:italic>\n operon reporter systems to differentially label pathogens and track intracellular replication, we confirmed T4SS-dependent intracellular growth of\n B. neotomae<\/jats:named-content>\n in macrophage cell lines. Furthermore,\n B. neotomae<\/jats:named-content>\n exhibited early endosomal (LAMP-1) and late endoplasmic reticulum (calreticulin)-associated phagosome maturation. These findings recapitulate prior observations for human-pathogenic\n Brucella<\/jats:named-content>\n spp. In addition, during coinfection experiments with\n Legionella pneumophila<\/jats:named-content>\n , we found that defective intracellular replication of a\n B. neotomae<\/jats:named-content>\n T4SS\n virB4<\/jats:italic>\n mutant was rescued and baseline levels of intracellular replication of wild-type\n B. neotomae<\/jats:named-content>\n were significantly stimulated by coinfection with wild-type but not T4SS mutant\n L. pneumophila<\/jats:named-content>\n . Using confocal microscopy, it was determined that intracellular colocalization of\n B. neotomae<\/jats:named-content>\n and\n L. pneumophila<\/jats:named-content>\n was required for rescue and that colocalization came at a cost to\n L. pneumophila<\/jats:named-content>\n fitness. These findings were not completely expected based on known temporal and qualitative differences in the intracellular life cycles of these two pathogens. Taken together, we have developed a new system for studying\n in vitro<\/jats:italic>\n Brucella<\/jats:named-content>\n pathogenesis and found a remarkable T4SS-dependent interplay between\n Brucella<\/jats:named-content>\n and\n Legionella<\/jats:named-content>\n during macrophage coinfection.\n <\/jats:p>","DOI":"10.1128\/iai.00991-16","type":"journal-article","created":{"date-parts":[[2017,3,7]],"date-time":"2017-03-07T01:40:52Z","timestamp":1488850852000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Promotion and Rescue of Intracellular Brucella neotomae Replication during Coinfection with Legionella pneumophila"],"prefix":"10.1128","volume":"85","author":[{"given":"Yoon-Suk","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Kirby","sequence":"additional","affiliation":[{"name":"Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Brucella infection and undulant fever in man","author":"Dalrymple-Champneys W","year":"1960","unstructured":"Dalrymple-Champneys W . 1960. 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