{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T21:49:57Z","timestamp":1781214597695,"version":"3.54.1"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Interleukin-10 (IL-10) has been implicated in susceptibility to genital chlamydial infection and the development of tubal pathologies. IL-10 limitation also resulted in the rapid elicitation of immune responses against<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>, and decreased levels of IL-10 correlated with protective anti-<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>immunity. To investigate the molecular basis for these effects, we compared the reproductive pathologies and fertility rates in<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>-infected wild-type (WT) and IL-10-knockout (IL-10<jats:sup>\u2212\/\u2212<\/jats:sup>) mice; we also analyzed the expression of the Toll-like receptor (TLR)\/interleukin-1 receptor (IL-1R) superfamily, IL-1\u03b2 production, NLRP3 inflammasome assembly and activation, and the immunostimulatory capacity and apoptotic predilection of<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>-exposed dendritic cells (DCs) from WT and IL-10<jats:sup>\u2212\/\u2212<\/jats:sup>mice. Our results revealed that, in addition to the rapid clearance of infection, genitally infected IL-10<jats:sup>\u2212\/\u2212<\/jats:sup>mice were protected from tubal pathologies and infertility, whereas WT (IL-10<jats:sup>+\/+<\/jats:sup>) mice were not.<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>-pulsed IL-10<jats:sup>\u2212\/\u2212<\/jats:sup>DCs expressed larger numbers of TLR4\/IL-1R molecules and had enhanced IL-1\u03b2 production. In addition, NLRP3 inflammasome assembly was suppressed in IL-10<jats:sup>\u2212\/\u2212<\/jats:sup>DCs through the inhibition of the P2X purinoceptor 7 (P2X7) receptor (P2X7R), an ATP-gated ion channel, and a decrease in intracellular Ca<jats:sup>2+<\/jats:sup>levels, which inhibited DC apoptosis. Thus, the potent immunostimulatory capacity of IL-10-deficient DCs is due, at least in part, to the suppression of the intracellular inflammasome assembly, which prevents DC apoptosis, allowing efficient antigen presentation. The results indicate that IL-10 deficiency enables efficient antigen presentation by DCs for rapid and enhanced immune activation against<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>, which results in rapid microbial clearance, which prevents tubal pathologies during infection. Our finding has important implications for the induction of protective immunity against<jats:named-content content-type=\"genus-species\">Chlamydia<\/jats:named-content>and other infectious and noninfectious diseases by vaccines.<\/jats:p>","DOI":"10.1128\/iai.00993-15","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T02:08:54Z","timestamp":1442282934000},"page":"4662-4672","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Interleukin-10 Modulates Antigen Presentation by Dendritic Cells through Regulation of NLRP3 Inflammasome Assembly during Chlamydia Infection"],"prefix":"10.1128","volume":"83","author":[{"given":"Yusuf","family":"Omosun","sequence":"first","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"},{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle","family":"McKeithen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"},{"name":"Department of Biology, Clark Atlanta University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khamia","family":"Ryans","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"},{"name":"Department of Biology, Clark Atlanta University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Kibakaya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uriel","family":"Blas-Machado","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Georgia College of Veterinary Medicine, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Neurology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Gang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Neurology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Eko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Black","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Igietseme","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"},{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biochemistry, and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA"},{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"834","article-title":"Prevalence of Chlamydia trachomatis genital infection among persons aged 14-39 years\u2014United States, 2007-2012","volume":"63","author":"Torrone E","year":"2014","unstructured":"Torrone E, Papp J, Weinstock H. 2014. 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