{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:55:43Z","timestamp":1791255343304,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Children's Research Centre, Dublin Ireland"},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["092530\/Z\/10\/Z"],"award-info":[{"award-number":["092530\/Z\/10\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Fund for Scientific Research","award":["WELBIO-CR-2015A-05"],"award-info":[{"award-number":["WELBIO-CR-2015A-05"]}]},{"name":"NIH","award":["GM080602"],"award-info":[{"award-number":["GM080602"]}]},{"name":"European Research Council","award":["693630"],"award-info":[{"award-number":["693630"]}]},{"DOI":"10.13039\/501100000296","name":"British Skin Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000296","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            skin infection is a frequent and recurrent problem in children with the common inflammatory skin disease atopic dermatitis (AD).\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            colonizes the skin of the majority of children with AD and exacerbates the disease. The first step during colonization and infection is bacterial adhesion to the cornified envelope of corneocytes in the outer layer, the stratum corneum. Corneocytes from AD skin are structurally different from corneocytes from normal healthy skin. The objective of this study was to identify bacterial proteins that promote the adherence of\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            to AD corneocytes.\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            strains from clonal complexes 1 and 8 were more frequently isolated from infected AD skin than from the nasal cavity of healthy children. AD strains had increased ClfB ligand binding activity compared to normal nasal carriage strains. Adherence of single\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            bacteria to corneocytes from AD patients\n            <jats:italic>ex vivo<\/jats:italic>\n            was studied using atomic force microscopy. Bacteria expressing ClfB recognized ligands distributed over the entire corneocyte surface. The ability of an isogenic ClfB-deficient mutant to adhere to AD corneocytes compared to that of its parent clonal complex 1 clinical strain was greatly reduced. ClfB from clonal complex 1 strains had a slightly higher binding affinity for its ligand than ClfB from strains from other clonal complexes. Our results provide new insights into the first step in the establishment of\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            colonization in AD patients. ClfB is a key adhesion molecule for the interaction of\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            with AD corneocytes and represents a target for intervention.\n          <\/jats:p>","DOI":"10.1128\/iai.00994-16","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T00:39:29Z","timestamp":1491266369000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Clumping Factor B Promotes Adherence of Staphylococcus aureus to Corneocytes in Atopic Dermatitis"],"prefix":"10.1128","volume":"85","author":[{"given":"Orla M.","family":"Fleury","sequence":"first","affiliation":[{"name":"Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maeve A.","family":"McAleer","sequence":"additional","affiliation":[{"name":"Clinical Medicine, Trinity College Dublin, Dublin, Ireland"},{"name":"National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland"},{"name":"Paediatric Dermatology, Our Lady's Children's Hospital, Crumlin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Feuillie","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, Universit\u00e9 Catholique de Louvain, Louvain-la-Neuve, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Formosa-Dague","sequence":"additional","affiliation":[{"name":"Institute of Life Sciences, Universit\u00e9 Catholique de Louvain, Louvain-la-Neuve, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Sansevere","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9sir\u00e9e E.","family":"Bennett","sequence":"additional","affiliation":[{"name":"Epidemiology and Molecular Biology Unit, Temple Street Children's University Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisling M.","family":"Towell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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